Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region, Ministry of Education, Institute of Agro-Bioengineering and College of Life Sciences, Guizhou University, Guiyang, 550025, China.
Biotechnol Lett. 2023 Aug;45(8):939-953. doi: 10.1007/s10529-023-03396-6. Epub 2023 May 27.
White rot fungi is a kind of filamentous fungi which can degrade lignin, hemicellulose and cellulose effectively. In this study, a wild white rot fungi collected from Pingba Town, Bijie City of China was identified as Coprinellus disseminatus (fruiting body) based on morphological and molecular identification. The mycelium of C. disseminatus cultured in the medium supplemented xylan as carbon showed the higher xylanase (XLE) and cellulase (CLE) activity. Further, the activities of tissue degradation-related enzymes including XLE, CLE, acetyl xylanesterase (AXE) and α-L-arabinofuran glycosidase (α-L-AF) were determined after fermenting Eucommia ulmoides leaves by inoculating C. disseminatus mycelium. The results showed that the activities of XLE, CLE, AXE and α-L-AF of mycelium cultured in xylan-contained medium reached the maximum at 5 d after inoculation, which were 777.606 ± 4.248 U mL, 9.594 ± 0.008 U mL, 4.567 ± 0.026 U mL and 3.497 ± 0.10 U mL respectively. Also, the activities of AXE and α-L-AF both reached the maximum in C. disseminatus mycelium cultured in glucose-contained medium. By comparing the yield of E. ulmoides gum under different fermentation treatments, the extraction yield of E. ulmoides gum were 2.156 ± 0.031% and 2.142 ± 0.044% at 7 d and 14 d after fermentation with mycelium supplemented xylan as carbon source, which were significantly higher than other groups. This study provides a theoretical reference for the preparation of E. ulmoides gum by large-scale fermentation of E. ulmoides leaves with C. disseminatus.
白色腐烂真菌是一种可以有效降解木质素、半纤维素和纤维素的丝状真菌。在这项研究中，从中国毕节市平坝镇采集的一种野生白色腐烂真菌被鉴定为Coprinellus disseminatus（子实体），基于形态学和分子鉴定。在补充木聚糖作为碳源的培养基中培养的 C. disseminatus 菌丝显示出更高的木聚糖酶（XLE）和纤维素酶（CLE）活性。进一步，在接种 C. disseminatus 菌丝发酵杜仲叶后，测定了与组织降解相关的酶的活性，包括木聚糖酶（XLE）、纤维素酶（CLE）、乙酰木聚糖酯酶（AXE）和α-L-阿拉伯呋喃糖苷酶（α-L-AF）。结果表明，在含有木聚糖的培养基中培养的菌丝体在接种后 5 天达到 XLE、CLE、AXE 和 α-L-AF 的最大活性，分别为 777.606±4.248 U mL、9.594±0.008 U mL、4.567±0.026 U mL 和 3.497±0.10 U mL。此外，在含有葡萄糖的培养基中培养的 C. disseminatus 菌丝体的 AXE 和 α-L-AF 活性均达到最大值。通过比较不同发酵处理下杜仲胶的产率，以木聚糖为碳源补充菌丝体发酵 7 d 和 14 d 后杜仲胶的提取率分别为 2.156±0.031%和 2.142±0.044%，显著高于其他组。本研究为利用 C. disseminatus 大规模发酵杜仲叶制备杜仲胶提供了理论参考。
