Zhao Liang, Hu Yang, Ji Qian-Yu, Gong Li-Xue, Lu Meng-Jiao, Yu Xue-Na, Jin Zhuo-Shuai, Zhou Min, Dai Xue-Lei, Xiao Shun-Yuan, Jiang Yu, Wen Ying-Qiang
State Key Laboratory of Crop Stress Resistance and High-Efficiency Production, College of Horticulture, Northwest A&F University, Yangling Shaanxi 712100, China.
Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture and Rural Affairs, Yangling Shaanxi 712100, China.
Hortic Res. 2025 Jun 10;12(9):uhaf146. doi: 10.1093/hr/uhaf146. eCollection 2025 Sep.
Grapevine powdery mildew (GPM), caused by , poses a significant threat to all green grapevine tissues, leading to substantial economic losses in viticulture. Traditional grapevine cultivars derived from are highly susceptible to GPM, whereas the wild Chinese accession Baishui-40 (BS-40) of var. exhibits robust resistance. To illuminate the genetic basis of resistance, we sequenced and assembled the chromosome-level genome of 'BS-40', achieving a total mapped length of 578.6 Mb distributed across nineteen chromosomes. A comprehensive annotation identified 897 nucleotide-binding leucine-rich repeat (NLR) genes in the 'BS-40' genome, which exhibited high sequence similarity across genomes. 284 of these NLR genes were differentially expressed upon GPM infection. A hybrid population of 'BS-40' and was constructed and 195 progenies were whole-genome re-sequenced. A new GPM-resistant locus, designated , located within the 0.74-1.23 Mb region on chromosome 1 was identified using genome-wide association study, population selection, and QTL analysis. Recombinant events indicated that an NLR gene cluster between 1 045 489 and 1 089 719 bp on chromosome 1 is possibly the key contributor to GPM resistance in 'BS-40'. Based on an SNP within this region, a dCAPS marker was developed that can predict the GPM resistance in 'BS-40'-derived materials with 99.4% accuracy in the progenies of 'BS-40' and . This chromosome-level genome assembly of var. provides a valuable resource not only for grapevine evolution, genetic analysis, and pan-genome studies but also a new locus as a promising target for GPM-resistant breeding in grapevine.
葡萄白粉病（GPM）由[病原体名称缺失]引起，对葡萄藤的所有绿色组织构成重大威胁，给葡萄栽培带来巨大经济损失。源自[亲本品种名称缺失]的传统葡萄品种对GPM高度敏感，而中国野生种‘白水-40’（BS-40）表现出较强抗性。为阐明其抗性的遗传基础，我们对‘BS-40’的染色体水平基因组进行了测序和组装，总映射长度为578.6 Mb，分布在19条染色体上。全面注释在‘BS-40’基因组中鉴定出897个核苷酸结合富含亮氨酸重复序列（NLR）基因，这些基因在[多个品种名称缺失]基因组间表现出高度序列相似性。其中284个NLR基因在GPM感染后差异表达。构建了‘BS-40’与[另一亲本品种名称缺失]的杂交群体，并对195个后代进行了全基因组重测序。通过全基因组关联研究、群体选择和QTL分析，在1号染色体上0.74 - 1.23 Mb区域鉴定出一个新的抗GPM位点，命名为[位点名称缺失]。重组事件表明，1号染色体上1 045 489至1 089 719 bp之间的一个NLR基因簇可能是‘BS-40’抗GPM的关键因素。基于该区域内的一个SNP，开发了一种dCAPS标记，可在‘BS-40’与[另一亲本品种名称缺失]的后代中以99.4%的准确率预测源自‘BS-40’材料的GPM抗性。‘BS-40’的这种染色体水平基因组组装不仅为葡萄进化、遗传分析和泛基因组研究提供了宝贵资源，也为葡萄抗GPM育种提供了一个有前景的新靶点[位点名称缺失]。
