Dowbaj Anna M, Kohler Timo N, Cordero-Espinoza Lucía, Hollfelder Florian, Huch Meritxell
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden 01307, Germany.
Wellcome Trust-Medical Research Council Stem Cell Institute Cambridge, Cambridge CB2 1QR, UK; Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, UK.
STAR Protoc. 2023 Jun 2;4(2):102333. doi: 10.1016/j.xpro.2023.102333.
Within the peri-portal region of the adult liver, portal fibroblasts exist in close proximity to epithelial ductal/cholangiocyte cells. However, the cellular interactions between them are poorly understood. Here, we provide two co-culture techniques to incorporate liver portal mesenchyme into ductal cell organoids, which recapitulate aspects of their cellular interactions in vitro. We integrate several techniques from mesenchyme isolation and expansion to co-culture by microfluidic cell co-encapsulation or 2D-Matrigel layer. The protocol is easily adaptable to other cells from other organs. For complete information on the generation and use of this protocol, please refer to Cordero-Espinoza et al..
在成体肝脏的门周区域，门周成纤维细胞与上皮导管/胆管细胞紧密相邻。然而，它们之间的细胞相互作用却知之甚少。在此，我们提供了两种共培养技术，将肝门间充质整合到导管细胞类器官中，从而在体外重现它们细胞相互作用的某些方面。我们整合了从间充质分离、扩增到通过微流控细胞共包封或二维基质胶层进行共培养的多种技术。该方案很容易适用于来自其他器官的其他细胞。有关此方案生成和使用的完整信息，请参考科尔德罗 - 埃斯皮诺萨等人的研究。
