Peña-Ramos Omar, Zhou Zheng
Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX, USA.
Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX, USA.
STAR Protoc. 2023 Jun 3;4(2):102332. doi: 10.1016/j.xpro.2023.102332.
In metazoans, the acidification of the phagosomal lumen is essential for the efficient degradation of cargoes. Here, we present a protocol for measuring the rate of acidification inside phagosomal lumen containing apoptotic cells in living C. elegans embryos. We describe steps for generating a worm population, selecting embryos, and mounting embryos on agar pads. We then detail live imaging of embryos and data analysis. This protocol is applicable to any organism in which real-time fluorescence imaging can be performed. For complete details on the use and execution of this protocol, please refer to Pena-Ramos et al. (2022)..
在后生动物中，吞噬体腔的酸化对于有效降解吞噬的物质至关重要。在此，我们展示了一种用于测量活的秀丽隐杆线虫胚胎中含有凋亡细胞的吞噬体腔内酸化速率的方法。我们描述了生成虫群、选择胚胎以及将胚胎固定在琼脂垫上的步骤。然后，我们详细介绍了胚胎的实时成像和数据分析。该方法适用于任何能够进行实时荧光成像的生物体。有关此方法的使用和执行的完整详细信息，请参考佩纳 - 拉莫斯等人（2022年）的文献。
