舌下预防性给予载有 OVA 的 MSC 衍生外泌体以预防过敏致敏。
Sublingual prophylactic administration of OVA-loaded MSC-derived exosomes to prevent allergic sensitization.
机构信息
Department of Immunology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Cancer, Petroleum and Environmental Pollutants Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Immunology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
出版信息
Int Immunopharmacol. 2023 Jul;120:110405. doi: 10.1016/j.intimp.2023.110405. Epub 2023 Jun 2.
AIM
This study evaluated the immunomodulatory and delivery potential of adipose tissue-isolated MSC-derived exosomes as a prophylactic regimen through a sublingual route in the ovalbumin (OVA)-induced allergic asthma murine model.
MATERIAL AND METHODS
Balb/c mice received 10 μg/dose of OVA-enriched MSC-derived exosomes as a prophylactic regimen in six doses during three weeks, and then OVA sensitization was conducted through intraperitoneal and aerosol administration of allergen. The total cells and eosinophils counted in nasal lavage fluid (NALF) and lung tissues were assessed for histopathological analysis. In addition, the secretion of IFN-γ, IL-4, and TGF-β by spleen cells and serum OVA-specific IgE levels were measured via ELISA.
RESULTS
Significant reduction in the IgE levels and IL-4 production, along with elevated TGF-β levels, were observed. Also, limited cellular infiltrations and perivascular and peribronchiolar inflammation in the lung tissues and normal total numbers of cells and eosinophils in the NALF were reported.
CONCLUSION
Prophylactic regimen using OVA-enriched MSC-derived exosomes modulated immune responses and inhibited allergic OVA sensitization.
目的
本研究通过卵清蛋白（OVA）诱导的变应性哮喘小鼠模型，评估脂肪组织分离的 MSC 衍生的细胞外囊泡作为预防性方案通过舌下途径的免疫调节和递药潜力。
材料和方法
Balb/c 小鼠接受 10μg/dose 的 OVA 富集 MSC 衍生的细胞外囊泡作为预防性方案，分六剂在三周内给予，然后通过腹腔内和雾化给予过敏原进行 OVA 致敏。鼻洗液（NALF）和肺组织中总细胞和嗜酸性粒细胞计数用于组织病理学分析。此外，通过 ELISA 测量脾细胞分泌的 IFN-γ、IL-4 和 TGF-β以及血清 OVA 特异性 IgE 水平。
结果
观察到 IgE 水平和 IL-4 产生的显著降低，同时 TGF-β 水平升高。此外，肺组织中细胞浸润和血管周围及支气管周围炎症有限，NALF 中的总细胞和嗜酸性粒细胞数量正常。
结论
使用 OVA 富集的 MSC 衍生的细胞外囊泡的预防性方案调节了免疫反应并抑制了变应原 OVA 致敏。
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