Shimizu T, Ishikawa Y, Tsurumi H, Hibi S, Takakura R, Morishima Y, Hasegawa I, Goto S
Vox Sang. 1986;50(4):203-7. doi: 10.1111/j.1423-0410.1986.tb04881.x.
If leukocyte- and platelet-poor red cells have been processed and stored in closed bags, their delivery, especially on holidays, will be easy. The modified warm-centrifuge method was applied to a quadruple bag prepared for such purpose. Red cells were processed in the first bag by the centrifugation of whole blood. The supernatant plasma was transferred to the second bag. A phosphate buffer in the third bag was introduced into red cells and diluted red cells were then incubated at 37 degrees C for 1 h in an inverted position. After centrifugation, the red cell phase below the buffy coat layer was isolated using a Biotest separation apparatus and stored in the fourth bag (containing a preservative with adenine, phosphate, glucose and 0.9% NaCl solution) for 28 days at 4 degrees C. Ninety-eight percent of the leukocyte and 97% of platelets in whole blood unit were removed with a red cell recovery of 86%. Biochemical changes in stored red cells were similar to those of conventional buffy coat-removed red cells suspended in the same preservative, except for rapid decreases in 2,3-diphosphoglycerate levels. Since processing buffy coat-removed red cells containing few leukocytes and platelets with the present method was simple and these units might be stored for over 14 days with minimum cell damage, we suggest that the present method is useful way of processing leukocyte- and platelet-poor red cells in blood centers.
如果已处理的少白细胞和少血小板红细胞保存在密闭袋中，那么它们的运送会很容易，尤其是在节假日期间。改良的温离心法应用于为此目的准备的四联袋。红细胞在第一个袋中通过全血离心进行处理。上清血浆转移至第二个袋中。将第三个袋中的磷酸盐缓冲液加入红细胞中，然后将稀释后的红细胞倒置在37℃孵育1小时。离心后，使用Biotest分离装置分离白膜层以下的红细胞相，并在4℃下保存在第四个袋中（含有含腺嘌呤、磷酸盐、葡萄糖和0.9%氯化钠溶液的保存液）28天。全血单位中98%的白细胞和97%的血小板被去除，红细胞回收率为86%。储存红细胞的生化变化与悬浮在相同保存液中的传统去白膜层红细胞相似，只是2,3-二磷酸甘油酸水平迅速下降。由于用本方法处理含少量白细胞和血小板的去白膜层红细胞很简单，且这些单位可在细胞损伤最小的情况下储存超过14天，我们认为本方法是血站处理少白细胞和少血小板红细胞的一种有用方法。
