Day John C, Gweon Hyun S, Post Rory J
Centre for Ecology and Hydrology (CEH) Wallingford, Wallingford, Oxfordshire, UK.
School of Natural Sciences and Psychology, Liverpool John Moores University, Liverpool, UK.
Mitochondrial DNA B Resour. 2016 Nov 22;1(1):799-801. doi: 10.1080/23802359.2016.1209091.
The complete mitochondrial genome of the European blackfly, Meigen, 1818 was sequenced using a combined Illumina and Sanger sequencing approach. Using the known sequence of Skuse, 1889 (Chironomidae) homologous NGS reads were identified and assembled. The genome is 15,367 bp in length and includes 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and a control region. Gene order resembles that of the ancestral dipteran gene arrangement. The base composition of the genome is A (37.6%), T (35.3%), C (15.8%) and G (11.3%). The control region between 12S rRNA and tRNAIle is composed of 362 bp with no obvious repetitive motifs.
采用Illumina测序和桑格测序相结合的方法，对欧洲蚋（Meigen，1818）的完整线粒体基因组进行了测序。利用已知的斯库斯蚋（Skuse，1889，摇蚊科）序列，鉴定并组装了同源的二代测序（NGS）读段。该基因组长度为15367 bp，包括13个蛋白质编码基因、2个核糖体RNA基因、22个转运RNA基因和一个控制区。基因排列顺序与祖先双翅目基因排列相似。基因组的碱基组成为：A（37.6%）、T（35.3%）、C（15.8%）和G（11.3%）。12S rRNA和tRNAIle之间的控制区由362 bp组成，没有明显的重复基序。
