染色体外环状DNA复合物的出现作为小鼠胚胎早期发育中细胞分化的最早信号之一：（小鼠胚胎/畸胎瘤/云母压制吸附/环状DNA复合物/DNA重排）

Emergence of the Extrachromosomal Circular DNA Complexes as One of the Earliest Signals of Cellular Differentiation in the Early Development of Mouse Embryo: (mouse embryo/teratocarcinoma/mica-press-adsorption/circular DNA complex/DNA rearrangement).

作者信息

Yamagishi Hideo, Kunisada Takahiro, Iwakura Yoichiro, Nishimune Yoshitake, Ogiso Yoko, Matsushiro Aizo

机构信息

Department of Biophysics, Facuty of Science, Kyoto University, Kyoto 606, Japan.

Institute for Virus Research, Kyoto University, Kyoto 606, Japan.

出版信息

Dev Growth Differ. 1983;25(6):563-569. doi: 10.1111/j.1440-169X.1983.00563.x.

DOI:10.1111/j.1440-169X.1983.00563.x

PMID:37282129

Abstract

A small number of mouse embryos and embryonal carcinoma cells were pressed by mica sheet; then the extruded DNA complexes were adsorbed to mica and processed for electron microscopy. Extrachromosomal circular DNA complexes longer than 1 μm emerged during the compaction process of mouse embryos and during the differentiation of embryonal carcinoma cells induced with retinoic acid. These DNA molecules are discussed as possible products of developmental gene rearrangements occurring in the chromosomal DNA.

摘要

用云母片挤压少量小鼠胚胎和胚胎癌细胞；然后将挤出的DNA复合物吸附到云母上并进行电子显微镜处理。在小鼠胚胎的压实过程以及视黄酸诱导的胚胎癌细胞分化过程中出现了长度超过1μm的染色体外环状DNA复合物。这些DNA分子被认为是染色体DNA中发生的发育基因重排的可能产物。