Drysdale Thomas A, Elinson Richard P
Department of Zoology, University of Toronto, Toronto, Ontario, Canada M5S 1A1.
Dev Growth Differ. 1992 Feb;34(1):51-59. doi: 10.1111/j.1440-169X.1992.00051.x.
The surface of the Xenopus tadpole contains three specialized, transient cell types; the ciliated, hatching gland, and cement gland cells. To distinguish whether the appearance of these cell types on the surface is due to induction of surface cells or due to migration of deep ectodermal cells into the surface, we transplanted labelled surface or deep cells to unlabelled hosts at early to mid-gastrulae. After raising the host to a tadpole (Stage 28), we examined the embryo's surface for ciliated, hatching gland, and cement gland cells, and observed which cells were labelled. We find that all ciliated cells move into the surface from the deep ectodermal layer along with other cells of unknown function. Hatching gland cells arise by induction of surface cells as do the majority of cement gland cells. A few deep cells give rise to cement gland cells. Therefore, migration of deep cells to the surface and localized induction of surface cells contribute to the final surface patterning of the Xenopus tadpole.
非洲爪蟾蝌蚪的体表包含三种特化的、短暂存在的细胞类型，即纤毛细胞、孵化腺细胞和黏液腺细胞。为了区分这些细胞类型在体表的出现是由于表面细胞的诱导作用，还是由于深层外胚层细胞迁移到表面，我们在原肠胚早期到中期将标记的表面细胞或深层细胞移植到未标记的宿主中。将宿主饲养至蝌蚪期（第28阶段）后，我们检查胚胎表面的纤毛细胞、孵化腺细胞和黏液腺细胞，并观察哪些细胞被标记。我们发现，所有纤毛细胞与其他功能未知的细胞一起从深层外胚层迁移到表面。孵化腺细胞和大多数黏液腺细胞一样，是由表面细胞的诱导产生的。少数深层细胞产生黏液腺细胞。因此，深层细胞向表面的迁移和表面细胞的局部诱导作用共同促成了非洲爪蟾蝌蚪最终的体表模式。
