Sangita Ilango, Vishwanath Sankar, Sadasiva Kadandale, Ramachandran Anupama, Thanikachalam Yashini, Ramya Vengidesh
Conservative Dentistry and Endodontics, Chettinad Dental College and Research Institute, Chennai, IND.
Conservative Dentistry and Endodontics, KSR (K.S. Rangasamy) Institute of Dental Science and Research, Erode, IND.
Cureus. 2023 May 7;15(5):e38677. doi: 10.7759/cureus.38677. eCollection 2023 May.
Aim The aim of this study is to compare and evaluate the antimicrobial efficacy of chlorhexidine, calcium hydroxide, and cetylpyridinium chloride against in the presence and absence of contamination with simulated periapical exudate at different time intervals. Methods Simulated wound exudate and cetylpyridinium chloride gel were prepared prior to testing. The test groups were divided into groups A and B based on the presence and absence of simulated wound exudate. They were further divided into four subgroups as follows: subgroup 1: calcium hydroxide; subgroup 2: 2% chlorhexidine gel; subgroup 3: 0.5% cetylpyridinium chloride gel; subgroup 4: 0.9% saline as control. was inoculated, and the test groups were evaluated at different time periods of six, 12, and 24 hours. Aliquots were then obtained and subjected to 10-fold serial dilutions. A total of 10 µl of individual samples was spread onto the nutrient agar medium using L-rod. The plates were then assessed for colony-forming units (CFU), and the values obtained were subjected to statistical analysis. Kolmogorov-Smirnov and Shapiro-Wilk normality tests were used to check whether the variables follow a normal distribution. For within-group comparison, the Friedman test and the Kruskal-Wallis test were used. For between-group comparison, the Mann-Whitney U test was used. Results Saline had the highest CFU values, while cetylpyridinium chloride had the lowest CFU values in both contaminated and non-contaminated groups. In all the conditions, the CFU values of cetylpyridinium chloride were significantly lowest compared to the other three groups. CFU values of the calcium hydroxide group were significantly high, followed by the chlorhexidine group when compared to cetylpyridinium chloride in both contaminated and non-contaminated groups. Conclusion Within the limitations of the current study, it can be concluded that cetylpyridinium chloride was the most effective intracanal medicament against than calcium hydroxide and chlorhexidine at varying time intervals, even in the presence of a periapical exudate. Thus, cetylpyridinium chloride can be considered an effective intracanal medicament for root canal disinfection.
目的 本研究旨在比较和评估洗必泰、氢氧化钙和西吡氯铵在有无模拟根尖周渗出物污染的情况下，于不同时间间隔对[具体细菌，原文未提及]的抗菌效果。方法 在测试前制备模拟伤口渗出液和西吡氯铵凝胶。根据有无模拟伤口渗出液将测试组分为A组和B组。它们进一步分为以下四个亚组：亚组1：氢氧化钙；亚组2：2%洗必泰凝胶；亚组3：0.5%西吡氯铵凝胶；亚组4：0.9%生理盐水作为对照。接种[具体细菌，原文未提及]，并在6小时、12小时和24小时的不同时间段对测试组进行评估。然后获取等分试样并进行10倍系列稀释。使用L棒将总共10μl的单个样品铺展在营养琼脂培养基上。然后评估平板上的菌落形成单位（CFU），并对获得的值进行统计分析。使用Kolmogorov-Smirnov和Shapiro-Wilk正态性检验来检查变量是否呈正态分布。对于组内比较，使用Friedman检验和Kruskal-Wallis检验。对于组间比较，使用Mann-Whitney U检验。结果 在污染组和未污染组中，生理盐水的CFU值最高，而西吡氯铵的CFU值最低。在所有条件下，与其他三组相比，西吡氯铵的CFU值显著最低。在污染组和未污染组中，与西吡氯铵相比，氢氧化钙组的CFU值显著较高，其次是洗必泰组。结论 在本研究的局限性内，可以得出结论，即使在存在根尖周渗出液的情况下，在不同时间间隔，西吡氯铵作为根管内药物对[具体细菌，原文未提及]的效果比氢氧化钙和洗必泰更有效。因此，西吡氯铵可被认为是一种有效的根管消毒根管内药物。
