Department of Biomaterials Science, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.
Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.
Dent Mater. 2021 Aug;37(8):1248-1259. doi: 10.1016/j.dental.2021.04.008. Epub 2021 May 8.
The objective of this study is to prepare new dental resins with a long-lasting antimicrobial activity. Specifically, this study evaluates an approach for controlling infection in root canals using sealers containing polyhydroxyethyl methacrylate trimethylolpropane trimethacrylate (polyHEMA/TMPT) particles loaded with cetylpyridinium chloride (CPC). In addition, the physical properties of sealers containing CPC-loaded polyHEMA/TMPT particles (CLP) are determined.
PolyHEMA/TMPT particles with 10 (10%-CLP) and 25wt.% CPC (25%-CLP) with different particle sizes were fabricated and incorporated in HEMA-based sealers. CPC-release profiles were evaluated over 14 days of immersion in water, followed by 14 days of storage and 14 days of water immersion. The antibacterial activity of these sealers against Enterococcus faecalis in dentinal tubules was assessed using a root-canal-infection model. Their sealing abilities were evaluated by fluid filtration and physical properties were tested according to the ISO 6876 standard. The long-term antibacterial activity of the cured sealer containing 25%-CLP (∼21μm particle diameter) was re-assessed after 1 year of storage.
After 28 days of immersion, 25%-CLP exhibited a higher and sustained CPC release unlike 10%-CLP. Residual bacteria in root dentinal tubules were eradicated by obturation with 25%-CLP-containing sealers. The incorporation of 25%-CLP (∼21μm) had no adverse effects on the sealing ability and physical properties of the sealer and resulted in long-term antibacterial activity.
The incorporation of CPC-loaded particles in HEMA resins yielded endodontic sealers with long-term bactericidal activity against E. faecalis in root canals. These sealers can potentially be used to prevent recurrent apical periodontitis.
本研究旨在制备具有持久抗菌活性的新型牙科树脂。具体而言，本研究评估了一种使用含有聚羟乙基甲基丙烯酸酯三羟甲基丙烷三甲基丙烯酸酯（polyHEMA/TMPT）载有十六烷基吡啶氯（CPC）颗粒的密封剂控制根管感染的方法。此外，还确定了含有 CPC 负载的 polyHEMA/TMPT 颗粒（CLP）的密封剂的物理性质。
制备了载有 10wt%（10%-CLP）和 25wt%（25%-CLP）CPC 的不同粒径的 polyHEMA/TMPT 颗粒，并将其掺入 HEMA 基密封剂中。在水中浸泡 14 天后，评估 CPC 释放曲线，然后在水中储存 14 天，再在水中浸泡 14 天。使用根管感染模型评估这些密封剂对牙本质小管内粪肠球菌的抗菌活性。通过流体过滤评估其密封能力，并根据 ISO 6876 标准测试物理性能。在储存 1 年后，重新评估含有 25%-CLP（直径约 21μm）的固化密封剂的长期抗菌活性。
浸泡 28 天后，25%-CLP 表现出更高且持续的 CPC 释放，而 10%-CLP 则不然。用含有 25%-CLP 的密封剂填充根管后，根管牙本质小管内的残留细菌被消灭。掺入 25%-CLP（直径约 21μm）对密封剂的密封能力和物理性能没有不良影响，并且具有长期的抗菌活性。
将 CPC 负载颗粒掺入 HEMA 树脂中，得到了具有长期杀菌活性的根管密封剂，可有效防治根管内粪肠球菌引起的根尖周炎。这些密封剂有望用于预防复发性根尖周炎。
