Ratnaningsih Hanim R, Noviana Zahra, Dewi Tirta Kumala, Loekito Supriyono, Wiyono Suryo, Gafur Abdul, Antonius Sarjiya
Department of Soil Science and Land Resources, Faculty of Agriculture, IPB University, Bogor 16680, Indonesia.
Research Center for Applied Microbiology, National Research and Innovation Agency (BRIN), Cibinong 16911, Indonesia.
Heliyon. 2023 May 26;9(6):e16306. doi: 10.1016/j.heliyon.2023.e16306. eCollection 2023 Jun.
The roles of plant growth-promoting rhizobacteria in promoting plant growth and soil health, including alteration in plant metabolism and production of phytohormones such as indole-3-acetic acid (IAA) and the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase, are indisputable. This study aimed to isolate and characterize beneficial bacteria isolated from the rhizosphere of pineapple from distinct stress-inducing habitats, including water excess-, herbicide-over-treated-, and pathogen-infected areas at PT Great Giant Foods located in Lampung, Indonesia. The isolated bacteria were screened based on IAA production and ACC deaminase activities. Six selected isolates produced IAA with concentrations of up to 36.93 mgL. The highest value belongs to sp. NCTB5I, followed by sp. CHTB 2C (13.13 mgL) and sp. CHTB 5B (6.65 mgL). All isolates were detected with ACC deaminase activities with sp. CHTJ 5H consuming 88% of ACC over 24 h, the highest among all. sp. CHTB 2C was detected with the highest ACC deaminase activity with the value of 13,370 nm α-ketobutyrate mgh. In another experiment, it was revealed that all selected isolates promote soybean growth. These bacteria are potential to be developed in the future as bioagents to promote plant growth, especially under stressful environmental conditions.
促进植物生长的根际细菌在促进植物生长和土壤健康方面的作用是无可争议的，包括改变植物新陈代谢以及产生植物激素，如吲哚 - 3 - 乙酸（IAA）和1 - 氨基环丙烷 - 1 - 羧酸（ACC）脱氨酶。本研究旨在从印度尼西亚楠榜省PT Great Giant Foods不同胁迫诱导栖息地的菠萝根际中分离和鉴定有益细菌，这些栖息地包括水分过多、除草剂过度处理和病原体感染区域。根据IAA产量和ACC脱氨酶活性对分离出的细菌进行筛选。六个选定的分离株产生的IAA浓度高达36.93 mg/L。最高值属于NCTB5I菌株，其次是CHTB 2C菌株（13.13 mg/L）和CHTB 5B菌株（6.65 mg/L）。所有分离株均检测到ACC脱氨酶活性，其中CHTJ 5H菌株在24小时内消耗了88%的ACC，在所有菌株中最高。CHTB 2C菌株检测到的ACC脱氨酶活性最高，值为13370 nmol α - 酮丁酸/mg h。在另一项实验中，结果表明所有选定的分离株都能促进大豆生长。这些细菌未来有潜力被开发为促进植物生长的生物制剂，特别是在胁迫环境条件下。
