Xiao Feng, Sun Gufeng, Zhu Hong, Guo Yun, Xu Fan, Hu Guowen, Huang Kai, Guo Hua
Department of Neurosurgery, The Second Affiliated Hospital of Nanchang University Nanchang 330006, Jiangxi, China.
Jiangxi Key Laboratory of Neurological Tumors and Cerebrovascular Diseases Nanchang 330006, Jiangxi, China.
Am J Cancer Res. 2023 May 15;13(5):2135-2154. eCollection 2023.
Cornichon family AMPA receptor auxiliary protein 4 (CNIH4) functions as an oncogene in several types of tumor. Nevertheless, the potential function of CNIH4 in lower-grade glioma (LGG) remains unclear. Pan-cancer analysis was implemented to comprehensively explore CNIH4 expression patterns and prognostic value in multiple cancers. Further, a systematic investigation of correlations between CNIH4 expression and clinical features, prognosis, biological functions, immune properties, genomic mutations, and treatment response was conducted, based on LGG expression patterns. CNIH4 expression levels and specific roles in LGG were also evaluated using experiments. Aberrant CNIH4 overexpression was detected in various tumors, and higher CNIH4 expression was linked with inferior prognosis, including in patients with LGG. Univariate and multivariate Cox regression analysis indicated that CNIH4 expression was an independent prognostic biomarker in patients with LGG. Our data also revealed that CNIH4 expression was strongly related to immune-associated signatures, immune cell infiltration, immune checkpoint genes, copy number alteration burden, tumor mutation burden, and treatment response in patients with LGG. experiments confirmed that CNIH4 was unusually elevated and crucial for cell proliferation, migration, invasion and cell cycle regulation in LGG. Together, our data validate CNIH4 may be an independent prognostic biomarker that could serve as a novel therapeutic target for improvement of prognosis in patients with LGG.
科尼雄家族AMPA受体辅助蛋白4（CNIH4）在多种类型的肿瘤中发挥癌基因的作用。然而，CNIH4在低级别胶质瘤（LGG）中的潜在功能仍不清楚。进行泛癌分析以全面探究CNIH4在多种癌症中的表达模式和预后价值。此外，基于LGG的表达模式，系统研究了CNIH4表达与临床特征、预后、生物学功能、免疫特性、基因组突变及治疗反应之间的相关性。还通过实验评估了CNIH4在LGG中的表达水平及具体作用。在各种肿瘤中均检测到CNIH4异常过表达，且较高的CNIH4表达与较差的预后相关，包括LGG患者。单因素和多因素Cox回归分析表明，CNIH4表达是LGG患者的独立预后生物标志物。我们的数据还显示，CNIH4表达与LGG患者的免疫相关特征、免疫细胞浸润、免疫检查点基因、拷贝数改变负担、肿瘤突变负担及治疗反应密切相关。实验证实，CNIH4在LGG中异常升高，且对细胞增殖、迁移、侵袭及细胞周期调控至关重要。总之，我们的数据证实CNIH4可能是一种独立的预后生物标志物，可作为改善LGG患者预后的新型治疗靶点。
