Hefei National Research Center for Physical Sciences at the Microscale, Chinese Academy of Sciences Key Laboratory of Urban Pollutant Conversion, Department of Environmental Science and Engineering, University of Science and Technology of China, Hefei 230026, China.
Department of Applied Chemistry, University of Science and Technology of China, Hefei 230026, China.
ACS Appl Mater Interfaces. 2023 Jun 21;15(24):29561-29567. doi: 10.1021/acsami.3c04416. Epub 2023 Jun 9.
Imaging nanoscale objects at interfaces is essential for revealing surface-tuned mechanisms in chemistry, physics, and life science. Plasmonic-based imaging, a label-free and surface-sensitive technique, has been widely used for studying the chemical and biological behavior of nanoscale objects at interfaces. However, direct imaging of surface-bonded nanoscale objects remains challenging due to uneven image backgrounds. Here, we present a new surface-bonded nanoscale object detection microscopy that eliminates strong background interference by reconstructing accurate scattering patterns at different positions. Our method effectively functions at low signal-to-background ratios, allowing for optical scattering detection of surface-bonded polystyrene nanoparticles and severe acute respiratory syndrome coronavirus 2 pseudovirus. It is also compatible with other imaging configurations, such as bright-field imaging. This technique complements existing methods for dynamic scattering imaging and broadens the applications of plasmonic imaging techniques for high-throughput sensing of surface-bonded nanoscale objects, enhancing our understanding of the properties, composition, and morphology of nanoparticles and surfaces at the nanoscale.
在界面上对纳米级物体进行成像对于揭示化学、物理和生命科学中的表面调谐机制至关重要。基于等离子体的成像作为一种无标记和表面敏感的技术，已被广泛用于研究纳米级物体在界面处的化学和生物行为。然而，由于不均匀的图像背景，直接对表面键合的纳米级物体进行成像仍然具有挑战性。在这里，我们提出了一种新的表面键合纳米级物体检测显微镜，通过在不同位置重建准确的散射模式来消除强背景干扰。我们的方法在低信噪比下有效工作，允许对表面键合的聚苯乙烯纳米颗粒和严重急性呼吸综合征冠状病毒 2 假病毒进行光学散射检测。它还与其他成像配置兼容，如明场成像。该技术补充了现有的动态散射成像方法，并拓宽了等离子体成像技术在表面键合纳米级物体高通量传感中的应用，增强了我们对纳米级纳米颗粒和表面的性质、组成和形态的理解。
