从埃塞俄比亚西北部贡德尔市家禽养殖场的鸡粪便中分离出的多药耐药和产超广谱β-内酰胺酶的肠杆菌科。

Multidrug-resistant and extended-spectrum beta-lactamase-producing Enterobacteriaceae isolated from chicken droppings in poultry farms at Gondar City, Northwest Ethiopia.

机构信息

Department of Medical Microbiology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.

出版信息

PLoS One. 2023 Jun 9;18(6):e0287043. doi: 10.1371/journal.pone.0287043. eCollection 2023.

DOI:10.1371/journal.pone.0287043

PMID:37294782

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10256222/

Abstract

BACKGROUND

The poultry sector is one of the largest and fastest-growing agricultural sub-sector, especially in developing countries like Ethiopia. In poultry production, poultry farmers use sub-optimum doses of antibiotics for growth promotion and disease prevention purpose. This indiscriminate use of antibiotics in poultry farms contributes to the emergence of antibiotic-resistant bacteria, which has adverse implications for public health. Therefore, this study is aimed to assess multidrug resistance and extended-spectrum beta-lactamase-producing Enterobacteriaceae from chicken droppings in poultry farms.

METHODS

A total of 87 pooled chicken-dropping samples were collected from poultry farms from March to June 2022. Samples were transported with buffered peptone water. Selenite F broth was used for the enrichment and isolation of Salmonella spp. Isolates were cultured and identified by using MacConkey agar, Xylose lysine deoxycholate agar, and routine biochemical tests. Kirby-Bauer disk diffusion technique and combination disk test were used for antibiotic susceptibility testing and confirmation of extended-spectrum beta-lactamase production, respectively. Data were entered using Epi-data version 4.6 and then exported to SPSS version 26 for analysis.

RESULT

Out of 87 pooled chicken droppings, 143 Enterobacteriaceae isolates were identified. Of these, E. coli accounts for 87 (60.8%), followed by Salmonella spp. 23 (16.1%), P. mirabilis 18 (12.6%) and K. pneumoniae 11 (7.7%). A high resistance rate was observed for ampicillin 131 (91.6%), followed by tetracycline 130 (90.9), and trimethoprim-sulfamethoxazole 94 (65.7%). The overall multidrug resistance rate was 116/143 (81.1%; 95% CI: 74.7-87.5). A total of 12/143 (8.4%; CI: 3.9-12.9) isolates were extended-spectrum beta-lactamase producers, with 11/87 (12.6%) E. coli and 1/11 (9.1%) K. pneumoniae.

CONCLUSION AND RECOMMENDATIONS

High prevalence of multi-drug resistant isolates was observed. This study alarms poultry as a potential reservoir of extended-spectrum beta-lactamase-producing Enterobacteriaceae, which might shed and contaminate the environment through faecal matter. Prudent use of antibiotics should be implemented to manage antibiotic resistance in poultry production.

摘要

背景

家禽业是最大和发展最快的农业子行业之一，尤其是在埃塞俄比亚等发展中国家。在家禽生产中，家禽养殖户为了促进生长和预防疾病而使用低于最佳剂量的抗生素。这种在禽舍中滥用抗生素导致了抗药性细菌的出现，这对公共卫生产生了不利影响。因此，本研究旨在评估来自禽舍鸡粪中的多药耐药和产超广谱β-内酰胺酶肠杆菌科。

方法

本研究共采集了 2022 年 3 月至 6 月来自禽舍的 87 份鸡粪混合样本。样品用缓冲蛋白胨水运输。亚硒酸盐 F 肉汤用于增菌和分离沙门氏菌属。通过麦康凯琼脂、木糖赖氨酸去氧胆酸盐琼脂和常规生化试验对分离株进行培养和鉴定。使用 Kirby-Bauer 圆盘扩散技术和组合圆盘试验分别进行抗生素敏感性试验和产超广谱β-内酰胺酶的确认。数据使用 Epi-data 版本 4.6 输入，然后导出到 SPSS 版本 26 进行分析。

结果

在 87 份鸡粪中，共鉴定出 143 株肠杆菌科分离株。其中，大肠杆菌占 87 株（60.8%），其次是沙门氏菌属 23 株（16.1%）、奇异变形杆菌 18 株（12.6%）和肺炎克雷伯菌 11 株（7.7%）。对氨苄西林的耐药率最高为 131 株（91.6%），其次是四环素 130 株（90.9%）和甲氧苄啶-磺胺甲恶唑 94 株（65.7%）。总的多药耐药率为 116/143（81.1%；95%CI：74.7-87.5）。共有 12/143（8.4%；CI：3.9-12.9）株为产超广谱β-内酰胺酶，其中 11/87（12.6%）为大肠杆菌，1/11（9.1%）为肺炎克雷伯菌。