Bremmelgaard A, Stender S, Lorentzen J, Kjeldsen K
Arteriosclerosis. 1986 Jul-Aug;6(4):442-52. doi: 10.1161/01.atv.6.4.442.
To measure the flux of free and esterified cholesterol from plasma into abdominal aortic tissue that had severe atherosclerotic lesions, we intravenously injected two autologous plasma samples containing radioactive cholesterol into patients scheduled for reconstructive arterial surgery. After the injections, blood samples were collected for calculation of the exposure of arterial tissue to labeled free and esterified plasma cholesterol. When tissue specimens were removed a few days after the injection, the aortic influx was determined by the simultaneous use of two differently labeled species of cholesterol. The flux of free and esterified cholesterol into 51 tissue specimens from the abdominal aorta of 12 normocholesterolemic patients was 41 +/- 3 and 45 +/- 3 nmol X cm-2 X day-1 (mean +/- SE), respectively, with 7% to 30% hydrolysis and 8% to 24% esterification of the labeled sterols in the atherosclerotic tissue. The influx was up to 100 times greater than the influx into nonatherosclerotic ascending aorta previously measured in other patients. The cholesterol content of the atherosclerotic tissue corresponded to 16 +/- 3 months (mean +/- SE) of continuous cholesteryl ester influx. Unless counteracted by cholesteryl ester efflux from the plaque, this influx provides enough cholesteryl ester from plasma to cause rapid lesion progression.
为了测量游离胆固醇和酯化胆固醇从血浆进入患有严重动脉粥样硬化病变的腹主动脉组织的通量，我们对计划进行动脉重建手术的患者静脉注射了两份含有放射性胆固醇的自体血浆样本。注射后，采集血样以计算动脉组织对标记的游离和酯化血浆胆固醇的暴露量。在注射几天后取出组织标本时，通过同时使用两种不同标记的胆固醇来测定主动脉的流入量。12名血脂正常患者腹主动脉的51个组织标本中，游离胆固醇和酯化胆固醇的通量分别为41±3和45±3 nmol·cm⁻²·天⁻¹（平均值±标准误），动脉粥样硬化组织中标记固醇的水解率为7%至30%，酯化率为8%至24%。该通量比之前在其他患者中测量的非动脉粥样硬化升主动脉的通量高出多达100倍。动脉粥样硬化组织中的胆固醇含量相当于连续16±3个月（平均值±标准误）的胆固醇酯流入量。除非被斑块中的胆固醇酯流出所抵消，这种流入量会从血浆中提供足够的胆固醇酯，导致病变迅速进展。
