Stomatology Faculty, A.I. Yevdokimov Moscow State University of Medicine and Dentistry, 20, p. 1 Delegatskaya St., 127473 Moscow, Russia.
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry RAS, 16/10 Miklukho-Maklaya St., 117997 Moscow, Russia.
Int J Mol Sci. 2023 Jun 2;24(11):9678. doi: 10.3390/ijms24119678.
The emission of nanoscale particles from the surfaces of dental implants leads to the cumulative effect of particle complexes in the bone bed and surrounding soft tissues. Aspects of particle migration with the possibility of their involvement in the development of pathological processes of systemic nature remain unexplored. The aim of this work was to study protein production during the interaction of immunocompetent cells with nanoscale metal particles obtained from the surfaces of dental implants in the supernatants. The ability to migrate nanoscale metal particles with possible involvement in the formation of pathological structures, in particular in the formation of gallstones, was also investigated. The following methods were used: microbiological studies, X-ray microtomography, X-ray fluorescence analysis, flow cytometry, electron microscopy, dynamic light scattering, and multiplex immunofluorescence analysis. For the first time, titanium nanoparticles in gallstones were identified by X-ray fluorescence analysis and electron microscopy with elemental mapping. The multiplex analysis method revealed that the physiological response of the immune system cells, in particular neutrophils, to nanosized metal particles significantly reduced TNF-a production both through direct interaction and through double lipopolysaccharide-induced signaling. For the first time, a significant decrease in TNF-a production was demonstrated when supernatants containing nanoscale metal particles were co-cultured with proinflammatory peritoneal exudate obtained from the peritoneum of the C57Bl/6J inbred mice line for one day.
从牙种植体表面发射出的纳米级颗粒导致颗粒复合物在骨床和周围软组织中不断累积。颗粒迁移的各个方面及其可能参与全身病理性过程的发展仍未得到探索。本研究的目的是研究免疫活性细胞与从牙种植体表面获得的纳米级金属颗粒在细胞上清液中相互作用时的蛋白质产生情况。还研究了纳米级金属颗粒的迁移能力及其可能参与病理性结构形成的可能性，特别是胆石形成的可能性。采用了以下方法：微生物学研究、X 射线断层摄影术、X 射线荧光分析、流式细胞术、电子显微镜、动态光散射和多重免疫荧光分析。首次通过 X 射线荧光分析和电子显微镜及元素映射鉴定了胆石中的钛纳米颗粒。多重分析方法表明，免疫系统细胞，特别是中性粒细胞对纳米级金属颗粒的生理反应通过直接相互作用和通过双脂多糖诱导的信号显著降低 TNF-a 的产生。首次证明，当将含有纳米级金属颗粒的上清液与来自 C57Bl/6J 近交系小鼠腹膜的炎性腹膜渗出物在一天内共培养时，TNF-a 的产生显著降低。
