芸香科植物提取物、馏分及分离化合物的细胞毒性、抗溶血和抗氧化活性

Cytotoxic, Anti-Hemolytic, and Antioxidant Activities of L. (Rutaceae) Extract, Fractions, and Isolated Compounds.

作者信息

Elizondo-Luévano Joel H, Rodríguez-Garza Nancy E, Bazaldúa-Rodríguez Aldo F, Romo-Sáenz César I, Tamez-Guerra Patricia, Verde-Star María J, Gomez-Flores Ricardo, Quintanilla-Licea Ramiro

机构信息

Departamento de Química, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), San Nicolás de los Garza 66455, NL, Mexico.

Grupo de Enfermedades Infecciosas y Tropicales (e-INTRO), Instituto de Investigación Biomédica de Salamanca, Centro de Investigación de Enfermedades Tropicales de la Universidad de Salamanca (IBSAL-CIETUS), Facultad de Farmacia, Universidad de Salamanca (USAL), 37007 Salamanca, Spain.

出版信息

Plants (Basel). 2023 Jun 2;12(11):2203. doi: 10.3390/plants12112203.

DOI:10.3390/plants12112203

PMID:37299182

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10255231/

Abstract

is an herb used to treat various ailments, and its potential cytotoxic effects on different tumor cell lines have been extensively studied. The present study aimed to evaluate the cytotoxic activity of methanol extract (RCME), sub-partitions obtained from solvents of increasing polarity, and major compounds, as well as their hemolytic, anti-hemolytic, and antioxidant potential. The in vitro cytotoxic activity against the human hepatocarcinoma (HEP-G2) and the murine lymphoma cell line (L5178Y-R) was evaluated using the colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay, whereas selectivity indices (SIs) were determined by comparing cytotoxicity against normal African green monkey kidney cells (VERO) and human peripheral blood mononuclear cells (PBMC). Hemolytic and anti-hemolytic activities were evaluated on human erythrocytes. The most effective cytotoxic treatment was evaluated for nitric oxide release by J774A.1 macrophages. Antioxidant activity of material was also determined. Results showed that RCME produced significant ( < 0.05) cytotoxicity in HEP-G2 (IC = 1.79 µg/mL) and L5178Y-R (IC = 1.60 µg/mL) cells and exhibited high SIs (291.50 and 114.80, respectively). In addition, the -hexane fraction (RCHF) showed an IC of 18.31 µg/mL in HEP-G2 cells and an SI of 9.48 in VERO cells, whereas the chloroform fraction (RCCF) evidenced an IC of 1.60 µg/mL in L5178Y-R cells and an SI of 34.27 in PBMC cells. Chalepensin (CHL), rutamarin (RTM), and graveolin (GRV), which are major components of , showed high activity against L5178Y-R cells, with IC of 9.15, 15.13 and SI of 45.08 µg/mL, respectively. In addition, CHL, RTM, and GRV showed SIs of 24.76, 9.98, and 3.52, respectively, when compared with PBMC cells. RCME at concentrations of 125 µg/mL and 250 µg/mL, significantly ( < 0.05) decreased nitrite production in J774A.1 cells, when exposed to lipopolysaccharide. This study demonstrated that RCME showed significant cytotoxic activity against HEP-G2 and L5178Y-R cells, without affecting normal VERO, PBMC, and J774A.1 cells.

摘要

是一种用于治疗各种疾病的草药，其对不同肿瘤细胞系的潜在细胞毒性作用已得到广泛研究。本研究旨在评估甲醇提取物（RCME）、从极性递增的溶剂中获得的亚组分以及主要化合物的细胞毒性活性，以及它们的溶血、抗溶血和抗氧化潜力。使用比色法3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）还原试验评估对人肝癌细胞（HEP-G2）和鼠淋巴瘤细胞系（L5178Y-R）的体外细胞毒性活性，而选择性指数（SIs）则通过比较对正常非洲绿猴肾细胞（VERO）和人外周血单核细胞（PBMC）的细胞毒性来确定。对人红细胞评估溶血和抗溶血活性。通过J774A.1巨噬细胞评估最有效的细胞毒性处理对一氧化氮释放的影响。还测定了该物质的抗氧化活性。结果表明，RCME在HEP-G2（IC = 1.79 µg/mL）和L5178Y-R（IC = 1.60 µg/mL）细胞中产生显著（< 0.05）的细胞毒性，并表现出高SIs（分别为291.50和114.80）。此外，正己烷馏分（RCHF）在HEP-G2细胞中的IC为18.31 µg/mL，在VERO细胞中的SI为9.48，而氯仿馏分（RCCF）在L5178Y-R细胞中的IC为1.60 µg/mL，在PBMC细胞中的SI为34.27。作为该草药主要成分的查莱平（CHL）、鲁他马林（RTM）和格拉维林（GRV）对L5178Y-R细胞表现出高活性，IC分别为9.15、15.13和SI为45.08 µg/mL。此外，与PBMC细胞相比，CHL、RTM和GRV的SIs分别为24.76、9.98和3.52。当暴露于脂多糖时，浓度为125 µg/mL和250 µg/mL的RCME显著（< 0.05）降低J774A.1细胞中的亚硝酸盐产生。本研究表明，RCME对HEP-G2和L5178Y-R细胞表现出显著的细胞毒性活性，而不影响正常的VERO、PBMC和J774A.1细胞。