University of Kufa, Faculty of Science, Department of Biology, Iraq.
Arch Razi Inst. 2021 Sep 1;76(3):659-666. doi: 10.22092/ari.2021.355413.1681. eCollection 2021 Summer.
In China, Japan, and Korea, has been used in traditional medicine for thousands of years. Panax is a plant used as a general tonic or adaptogen for chronically ill patients. The current study evaluated the cytotoxicity of extract (PGE). Different cell lines (HCT-116, LNCaP, and normal cell line VERO) were treated with different inhibitory agentsat different concentrations (1000, 500, 250, 125, 62.5, and 31.25 µg/ml) as follows: G1 (Methanol extract, PGE), G2 (Doxorubicin, DOX), and G3 (Methanol extract +DOX, PDD). Each inhibitory agent group was used to treat the cancerous cell lines HCT-116, LNCaP, and normal cell line (VERO) to obtain IC50% by MTT assay. The inhibitory ability of the 1000 μg/ml PGE was significantly increased in all the three-cell lines compared with other concentrations. The recorded data revealed that the inhibition ability of PGE and Doxorubicin towards the HCT-116 cell line significantly increased compared with the other cell lines. The interaction between different PGE concentrations and cell lines showed that the 1000 μg/ml PEG had the highest inhibitory effects on HCT-116 compared with other combinations. The interaction between different DOX concentrations and different types of cell lines showed that the 1000 μg/ml DOX had the highest inhibitory effects on LNCap compared with other combinations. The PGD inhibition ability reflected a significantly higher difference toward the HCT-116 cell line as compared with other cell lines. IC50% is the concentrations (µg/ml) to kill 50% of cell line. It was calculated by MTT assay for three cell lines: HCT-116, LNCaP, and VERO. The rate of effectiveness of the inhibitory factors (PGE, DOX, and PGD) showed highly significant differences toward the cell line HCT-116 compared to the other cell lines. This indicates the safety of the PGE compound and its low toxicity toward normal cells, quite the opposite of cancer cells as compared to the common drug DOX and combined PGD (PGE+DOX). PGD combined with DOX (PGE + DOX) showed antagonistic results toward the HCT116, LNCaP, and VERO cell lines, while UDE combined with DOX (UDE+DOX) showed synergistic activity.
在中国、日本和韩国， 已被用于传统医学几千年。 是一种被用作慢性病患者的一般滋补品或适应原的植物。本研究评估了 提取物（PGE）的细胞毒性。不同的细胞系（HCT-116、LNCaP 和正常细胞系 VERO）用不同浓度的不同抑制剂（1000、500、250、125、62.5 和 31.25µg/ml）处理如下：G1（甲醇提取物，PGE）、G2（阿霉素，DOX）和 G3（甲醇提取物+DOX，PDD）。每个抑制剂组均用于治疗癌细胞系 HCT-116、LNCaP 和正常细胞系（VERO），通过 MTT 测定法获得 IC50%。与其他浓度相比，1000µg/ml PGE 在所有三种细胞系中的抑制能力显著增加。记录的数据显示，与其他细胞系相比，PGE 和阿霉素对 HCT-116 细胞系的抑制能力显著增加。不同 PGE 浓度与细胞系之间的相互作用表明，与其他组合相比，1000µg/ml PEG 对 HCT-116 的抑制作用最高。不同 DOX 浓度与不同类型细胞系之间的相互作用表明，与其他组合相比，1000µg/ml DOX 对 LNCap 的抑制作用最高。PGD 的抑制能力对 HCT-116 细胞系的差异明显高于其他细胞系。IC50%是杀死 50%细胞系所需的浓度（µg/ml）。通过 MTT 测定法对三种细胞系：HCT-116、LNCaP 和 VERO 进行了计算。抑制因子（PGE、DOX 和 PGD）的有效率对 HCT-116 细胞系的差异具有高度显著性，与其他细胞系相比。这表明 PGE 化合物的安全性及其对正常细胞的低毒性，与普通药物 DOX 和联合 PGD（PGE+DOX）形成鲜明对比。PGD 与 DOX（PGE+DOX）联合对 HCT116、LNCaP 和 VERO 细胞系表现出拮抗作用，而 UDE 与 DOX（UDE+DOX）联合表现出协同作用。
