Siquier-Dameto Gabriel, Boisnic Sylvie, Boadas-Vaello Pere, Verdú Enrique
Dameto Clinics International, 1171 VC Badhoevedorp, The Netherlands.
Research Group of Clinical Anatomy, Embryology and Neuroscience (NEOMA), Department of Medical Sciences, University of Girona, E-17003 Girona, Catalonia, Spain.
Polymers (Basel). 2023 May 24;15(11):2438. doi: 10.3390/polym15112438.
Solar radiation and environmental pollutants are factors that cause changes in the skin that trigger skin aging. The objective of the study is to evaluate the rejuvenating effects of a complex formed by hyaluronic acid supplemented with vitamins, amino acids and oligopeptides in explants of human skin. For this, surplus skin samples have been obtained from donors that have been resected and cultivated on slides with membrane inserts. The complex was administered to some skin explants and the percentage of cells with low, medium and high levels of melanin was evaluated as an indicator of the degree of pigmentation. Other skin segments were irradiated with UVA/UVB, then the product was administered on several slides and the levels of collagen, elastin, sulfated GAG and MMP1 were evaluated. The results show that the administration of the complex significantly reduces the percentage of skin cells with a high melanin content by 16%, and that in skin irradiated with UVA/UVB, there is a decrease in the content of collagen, elastin and sulfate GAGs, and the complex reverses this reduction without changing MMP1 levels. This suggests that the compound has anti-aging and depigmentation effects on the skin, giving a skin rejuvenation appearance.
太阳辐射和环境污染物是导致皮肤变化进而引发皮肤衰老的因素。本研究的目的是评估由透明质酸与维生素、氨基酸和寡肽组成的复合物对人皮肤外植体的焕肤效果。为此，已从供体获取多余的皮肤样本，这些样本经过切除后在带有膜插入物的载玻片上培养。将该复合物施用于一些皮肤外植体，并评估黑色素含量低、中、高的细胞百分比，以此作为色素沉着程度的指标。对其他皮肤片段进行UVA/UVB照射，然后在多个载玻片上施用该产品，并评估胶原蛋白、弹性蛋白、硫酸化糖胺聚糖和MMP1的水平。结果表明，施用该复合物可使黑色素含量高的皮肤细胞百分比显著降低16%，并且在接受UVA/UVB照射的皮肤中，胶原蛋白、弹性蛋白和硫酸化糖胺聚糖的含量会降低，而该复合物可逆转这种降低，且不改变MMP1水平。这表明该化合物对皮肤具有抗老化和色素沉着作用，呈现出皮肤焕肤的外观。
