Laboratory of Veterinary Internal Medicine II, School of Veterinary Medicine, Nippon Veterinary and Life Science University, Tokyo, Japan.
Open Vet J. 2023 May;13(5):604-612. doi: 10.5455/OVJ.2023.v13.i5.13. Epub 2023 May 14.
Plasma N-terminal pro-atrial natriuretic peptide (NT-proANP) and plasma N-terminal pro-B-type natriuretic peptide (NT-proBNP) concentrations may be affected by the hydration status.
This study aimed to evaluate the effect of dehydration on plasma NT-proANP and NT-proBNP concentrations in healthy dogs.
This prospective study included five clinically healthy dogs. Furosemide was administered intravenously at 2-4 mg/kg every 1-2 hours until completion of the dehydration model. The dehydration model was considered complete when weight loss was ≥5% and findings of dehydration on physical examination were observed. Plasma NT-proANP and NT-proBNP concentrations were compared at three-time points: before the dehydration model was created (point 1), at the completion of the dehydration model (point 2), and when dehydration was judged to have improved (point 3). Association between plasma NT-proANP and NT-proBNP concentrations, and each clinical variable (physical examination, blood pressure, blood chemistry, blood gases, and echocardiography) was assessed using linear regression analysis.
Plasma NT-proANP concentration decreased significantly from point 2 to point 1 ( < 0.05), whereas plasma NT-proBNP concentration showed a decreasing trend but did not differ significantly between points 1 and 2. Plasma NT-proANP concentration correlated significantly with body weight ( = 0.178) and plasma NT-proBNP concentration ( = 0.284) ( < 0.05, respectively), and plasma NT-proBNP concentration correlated significantly with electrolytes (sodium, = 0.439; potassium, = 0.444; and chloride, = 0.419), and echocardiographic parameters [diastolic left ventricular internal diameter (LVIDd) = 0.519; weight-standardized LVIDd, = 0.535] ( < 0.01, respectively).
The plasma NT-proANP concentrations decreased with dehydration. However, the plasma NT-proBNP concentration did not change with mild dehydration and reflected left ventricular morphology.
血浆 N 末端脑利钠肽前体（NT-proANP）和血浆 N 末端 pro-B 型利钠肽（NT-proBNP）浓度可能受水合状态的影响。
本研究旨在评估脱水对健康犬血浆 NT-proANP 和 NT-proBNP 浓度的影响。
这项前瞻性研究纳入了 5 只临床健康的犬。呋塞米以 2-4mg/kg 静脉内给药，每 1-2 小时 1 次，直至脱水模型完成。当体重减轻≥5%且体格检查发现脱水表现时，认为脱水模型完成。在 3 个时间点比较血浆 NT-proANP 和 NT-proBNP 浓度：创建脱水模型前（点 1）、脱水模型完成时（点 2）和判断脱水改善时（点 3）。使用线性回归分析评估血浆 NT-proANP 和 NT-proBNP 浓度与每个临床变量（体格检查、血压、血液化学、血气和超声心动图）之间的相关性。
与点 1 相比，点 2 时血浆 NT-proANP 浓度显著降低（<0.05），而 NT-proBNP 浓度呈下降趋势，但点 1 和点 2 之间无显著差异。血浆 NT-proANP 浓度与体重呈显著相关（r=0.178，<0.05），与血浆 NT-proBNP 浓度呈显著相关（r=0.284，<0.05），血浆 NT-proBNP 浓度与电解质（钠，r=0.439；钾，r=0.444；氯，r=0.419）和超声心动图参数[舒张期左心室内径（LVIDd），r=0.519；体重标准化的 LVIDd，r=0.535]呈显著相关（<0.01，分别）。
随着脱水，血浆 NT-proANP 浓度降低。然而，在轻度脱水时，血浆 NT-proBNP 浓度不变，反映了左心室形态。
