基于蛋白笼的结晶生物杂化材料。

Crystalline Biohybrid Materials Based on Protein Cages.

机构信息

Universität Hamburg, Department of Chemistry, Institute of Physical Chemistry, Hamburg, Germany.

The Hamburg Centre for Ultrafast Imaging, Hamburg, Germany.

出版信息

Methods Mol Biol. 2023;2671:361-386. doi: 10.1007/978-1-0716-3222-2_21.

DOI:10.1007/978-1-0716-3222-2_21

PMID:37308656

Abstract

Highly ordered superstructures of nanomaterials can be synthesized using protein cages as templates for the assembly of inorganic nanoparticles. Here, we describe in detail the creation of these biohybrid materials. The approach involves computational redesign of ferritin cages, followed by recombinant protein production and purification of the new variants. Metal oxide nanoparticles are synthesized inside the surface-charged variants. The composites are assembled using protein crystallization to yield highly ordered superlattices, which are characterized, for example, with small angle X-ray scattering. This protocol provides a detailed and comprehensive account on our newly established strategy for the synthesis of crystalline biohybrid materials.

摘要

高度有序的纳米材料超结构可以使用蛋白质笼作为模板来组装无机纳米粒子。在这里，我们详细描述了这些生物杂化材料的创建过程。该方法涉及对铁蛋白笼进行计算重新设计，然后进行重组蛋白的生产和新变体的纯化。金属氧化物纳米粒子在带电荷表面的变体内部合成。然后使用蛋白质结晶组装复合材料，得到高度有序的超晶格，例如用小角 X 射线散射进行表征。本方案提供了关于我们新建立的合成结晶生物杂化材料策略的详细和全面的说明。