甘蓝型油菜脂肪酸输出蛋白FAX1-1对生物产量、种子含油量和油质有贡献。
The Brassica napus fatty acid exporter FAX1-1 contributes to biological yield, seed oil content, and oil quality.
作者信息
Xiao Zhongchun, Tang Fang, Zhang Liyuan, Li Shengting, Wang Shufeng, Huo Qiang, Yang Bo, Zhang Chao, Wang Daojie, Li Qing, Wei Lijuan, Guo Tao, Qu Cunmin, Lu Kun, Zhang Yanfeng, Guo Liang, Li Jiana, Li Nannan
机构信息
Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, China.
Academy of Agricultural Sciences, Southwest University, Beibei, Chongqing, 400715, China.
出版信息
Biotechnol Biofuels. 2021 Sep 29;14(1):190. doi: 10.1186/s13068-021-02035-4.
BACKGROUND
In the oilseed crop Brassica napus (rapeseed), various metabolic processes influence seed oil content, oil quality, and biological yield. However, the role of plastid membrane proteins in these traits has not been explored.
RESULTS
Our genome-wide association study (GWAS) of 520 B. napus accessions identified the chloroplast membrane protein-localized FATTY ACID EXPORTER 1-1 (FAX1-1) as a candidate associated with biological yield. Seed transcript levels of BnaFAX1-1 were higher in a cultivar with high seed oil content relative to a low-oil cultivar. BnaFAX1-1 was localized to the plastid envelope. When expressed in Arabidopsis thaliana, BnaFAX1-1 enhanced biological yield (total plant dry matter), seed yield and seed oil content per plant. Likewise, in the field, B. napus BnaFAX1-1 overexpression lines (BnaFAX1-1-OE) displayed significantly enhanced biological yield, seed yield, and seed oil content compared with the wild type. BnaFAX1-1 overexpression also up-regulated gibberellic acid 4 (GA4) biosynthesis, which may contribute to biological yield improvement. Furthermore, oleic acid (C18:1) significantly increased in BnaFAX1-1 overexpression seeds.
CONCLUSION
Our results indicated that the putative fatty acid exporter BnaFAX1-1 may simultaneously improve seed oil content, oil quality and biological yield in B. napus, providing new approaches for future molecular breeding.
背景
在油料作物甘蓝型油菜（油菜籽）中，各种代谢过程影响种子油含量、油质和生物产量。然而，质体膜蛋白在这些性状中的作用尚未得到探索。
结果
我们对520份甘蓝型油菜种质进行全基因组关联研究（GWAS），确定定位于叶绿体膜蛋白的脂肪酸输出蛋白1-1（FAX1-1）是与生物产量相关的候选基因。相对于低油品种，高种子油含量品种中BnaFAX1-1的种子转录水平更高。BnaFAX1-1定位于质体包膜。在拟南芥中表达时，BnaFAX1-1提高了生物产量（全株干物质）、种子产量和单株种子油含量。同样，在田间，甘蓝型油菜BnaFAX1-1过表达系（BnaFAX1-1-OE）与野生型相比，生物产量、种子产量和种子油含量显著提高。BnaFAX1-1过表达还上调了赤霉素4（GA4）的生物合成，这可能有助于提高生物产量。此外，BnaFAX1-1过表达种子中的油酸（C18:1）显著增加。
结论
我们的结果表明，假定的脂肪酸输出蛋白BnaFAX1-1可能同时提高甘蓝型油菜的种子油含量、油质和生物产量，为未来分子育种提供了新途径。
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