Ahmad Ali, Li Wenhui, Zhang Hui, Wang Hao, Wang Pengfei, Jiao Yushun, Zhao Chenqi, Yang Guangsheng, Hong Dengfeng
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070 People's Republic of China.
Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070 People's Republic of China.
Mol Breed. 2023 Feb 8;43(2):11. doi: 10.1007/s11032-023-01355-7. eCollection 2023 Feb.
Ovule number (ON) produced during flower development determines the maximum number of seeds per silique and thereby affects crop productivity; however, the genetic basis of ON remains poorly understood in oilseed rape (). In this study, we genetically dissected the ON variations in a double haploid (DH) population and in natural population (NP) by linkage mapping and genome-wide association analysis. Phenotypic analysis showed that ON displayed normal distribution in both populations with the broad-sense heritability of 0.861 (DH population) and 0.930 (natural population). Linkage mapping identified 5 QTLs related to ON, including , , , , and . Genome-wide association studies (GWAS) revealed 214, 48, and 40 significant single-nucleotide polymorphisms (SNPs) by individually using the single-locus model GLM and the multiple-locus model MrMLM and FASTMrMLM. The phenotypic variation explained (PVE) by these QTLs and SNPs ranged from 2.00-17.40% to 5.03-7.33%, respectively. Integration of the results from both strategies identified four consensus genomic regions associated with ON from the chromosomes A03, A07, and A10. Our results preliminarily resolved the genetic basis of ON and provides useful molecular markers for plant yield improvement in .
The online version contains supplementary material available at 10.1007/s11032-023-01355-7.
花发育过程中产生的胚珠数（ON）决定了每角果种子的最大数量，从而影响作物产量；然而，油菜籽中ON的遗传基础仍知之甚少。在本研究中，我们通过连锁图谱分析和全基因组关联分析，对双单倍体（DH）群体和自然群体（NP）中的ON变异进行了遗传剖析。表型分析表明，ON在两个群体中均呈正态分布，广义遗传力分别为0.861（DH群体）和0.930（自然群体）。连锁图谱分析鉴定出5个与ON相关的QTL，包括 、 、 、 和 。全基因组关联研究（GWAS）分别使用单基因座模型GLM和多基因座模型MrMLM及FASTMrMLM，揭示了214、48和40个显著的单核苷酸多态性（SNP）。这些QTL和SNP解释的表型变异（PVE）分别为2.00 - 17.40%至5.03 - 7.33%。两种策略结果的整合确定了来自A03、A07和A10染色体的四个与ON相关的共有基因组区域。我们的结果初步解析了ON的遗传基础，并为油菜籽的植物产量改良提供了有用的分子标记。
在线版本包含可在10.1007/s11032-023-01355-7获取的补充材料。
