控制油菜（L.）色素积累的基因精细定位。
Fine mapping of genes controlling pigment accumulation in oilseed rape ( L.).
作者信息
Chen Daozong, Jin Qingdong, Pan Jianming, Liu Yi, Tang Yijia, E Yanrong, Xu Linshan, Yang Taihua, Qiu Jie, Chen Xiaodi, Wang Jing, Gong Deping, Ge Xianhong, Li Zaiyun, Cui Cheng
机构信息
College of Life Sciences, Ganzhou Key Laboratory of Greenhouse Vegetable, Gannan Normal University, Ganzhou, 341000 China.
National Key Laboratory of Crop Genetic Improvement, National Center of Oil Crop Improvement (Wuhan), College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070 People's Republic of China.
出版信息
Mol Breed. 2023 Mar 9;43(3):19. doi: 10.1007/s11032-023-01365-5. eCollection 2023 Mar.
UNLABELLED
Purple/red appearance is one of the common phenotypic variations in leaves, stems, and siliques of oilseed rape ( L.) but very rare in flowers. In this study, the causal genes for the purple/red traits in stems and flowers in two accessions of oilseed rape (DH_PR and DH_GC001, respectively) derived from the wide hybridization were fine mapped, and candidate genes were determined by methods combined with bulked segregant analysis (BSA) and RNA-seq analysis. Both traits of purple stem and red flowers were mapped to the locus as homologous genes ( and , respectively) belonging to the R2R3-MYB family Sequence comparisons of full-length allelic genes revealed several InDels and SNPs in intron 1 as well as exons, and completely different promoter region of and a 211 bp insertion was identified in the promoter region of of DH_GC001. Our results not only contribute to a better understanding of anthocyanin inheritance in , but also provide a useful toolbox for future breeding of cultivars with purple/red traits through the combination of different functional alleles and homologs.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s11032-023-01365-5.
未标注
紫色/红色外观是油菜（L.）叶片、茎和角果常见的表型变异之一，但在花中非常罕见。在本研究中，对两个源自远缘杂交的油菜材料（分别为DH_PR和DH_GC001）茎和花中紫色/红色性状的因果基因进行了精细定位，并通过混合分组分析法（BSA）和RNA测序分析相结合的方法确定了候选基因。紫色茎和红色花这两个性状均被定位到同一基因座，分别为属于R2R3-MYB家族的同源基因（和）。全长等位基因的序列比较揭示了内含子1以及外显子中的几个插入缺失（InDels）和单核苷酸多态性（SNPs），并且和的启动子区域完全不同，在DH_GC001的的启动子区域鉴定出一个211 bp的插入。我们的研究结果不仅有助于更好地理解油菜中花青素的遗传，还为未来通过不同功能等位基因和同源基因的组合培育具有紫色/红色性状的品种提供了一个有用的工具箱。
补充信息
在线版本包含可在10.1007/s11032-023-01365-5获取的补充材料。
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