Maksimovic Nela, Vidovic Vanja, Damnjanovic Tatjana, Jekic Biljana, Majkic Singh Nada, Simeunovic Slavko, Bozovic Dara Savic, Vidovic Stojko, Novakovic Ivana
Institute of Human Genetics, Faculty of Medicine, University of Belgrade, Serbia.
Faculty of Medicine, University of Banja Luka, Banja Luka, Bosnia and Herzegovina.
Arch Med Sci. 2021 Jan 8;19(3):593-599. doi: 10.5114/aoms/113174. eCollection 2023.
Positive regulatory domain containing 16 (PRDM16) protein represents the key regulator of brown adipose tissue (BAT) development. It induces brown fat phenotype and represses white adipose tissue specific genes through the association with -terminal binding co-repressor proteins (CtBP1 and CtBP2). In healthy adults presence of BAT has been associated with lower glucose, total cholesterol and low-density lipoprotein (LDL) cholesterol levels. Our aim was to analyze the association of gene (rs12409277) and gene (rs1561589) polymorphisms with body mass index (BMI), fasting glucose level and lipid profile of adolescents.
Our study included 295 healthy school children, 145 boys (49.2%) and 150 girls (50.8%), 15 years of age. Genotypes for the selected polymorphisms were detected by the real-time PCR method. Age, gender, height, weight, lipid profile (total cholesterol, high-density lipoprotein (HDL) cholesterol, LDL cholesterol, triglycerides) and fasting glucose levels were recorded.
We did not find a statistically significant association of rs12409277 and rs1561589 polymorphisms with BMI, fasting glucose and lipid profile of adolescents. We further analyzed the combined effect of the two SNPs and the statistical analysis showed that carriers of CT genotype of rs12409277 polymorphism and GG genotype of rs1561589 polymorphism had significantly lower total cholesterol ( = 0.001) and LDL cholesterol ( = 0.008) levels compared to all other groups of genotypes.
Our study suggests that rs12409277 and rs1561589 polymorphism might have an influence on total and LDL cholesterol levels in adolescents. Larger studies should be performed in order to confirm our results.
含16的正调控结构域（PRDM16）蛋白是棕色脂肪组织（BAT）发育的关键调节因子。它通过与C末端结合共抑制蛋白（CtBP1和CtBP2）结合来诱导棕色脂肪表型并抑制白色脂肪组织特异性基因。在健康成年人中，BAT的存在与较低的血糖、总胆固醇和低密度脂蛋白（LDL）胆固醇水平相关。我们的目的是分析基因（rs12409277）和基因（rs1561589）多态性与青少年体重指数（BMI）、空腹血糖水平和血脂谱的关联。
我们的研究纳入了295名15岁的健康在校儿童，其中145名男孩（49.2%）和150名女孩（50.8%）。通过实时PCR方法检测所选多态性的基因型。记录年龄、性别、身高、体重、血脂谱（总胆固醇、高密度脂蛋白（HDL）胆固醇、LDL胆固醇、甘油三酯）和空腹血糖水平。
我们未发现rs12409277和rs1561589多态性与青少年的BMI、空腹血糖和血脂谱之间存在统计学上的显著关联。我们进一步分析了这两个单核苷酸多态性（SNP）的联合效应，统计分析表明，与所有其他基因型组相比，rs12409277多态性的CT基因型携带者和rs1561589多态性的GG基因型携带者的总胆固醇（P = 0.001）和LDL胆固醇（P = 0.008）水平显著较低。
我们的研究表明，rs12409277和rs1561589多态性可能会影响青少年的总胆固醇和LDL胆固醇水平。需要进行更大规模的研究以证实我们的结果。
