Hanlon D P, Ferm V H
Environ Res. 1986 Aug;40(2):380-90. doi: 10.1016/s0013-9351(86)80113-x.
We have determined the concentration and chemical composition of arsenic in the blood of pregnant hamsters between 0.2 and 6 hr after an intraperitoneal injection of a teratogenic dose of radiolabeled sodium arsenate on the morning of the eighth day of gestation. Arsenic was present in plasma and red cells 0.20 hr postinjection. The plasma arsenic concentration reached a maximum of 220 mumole/kg blood near 0.5 hr postinjection. Plasma arsenic existed entirely as low-molecular-weight species. Both arsenite and dimethylarsinate (DMA) were present in plasma 0.20 hr postinjection, indicating that arsenate reduction and methylation of arsenic are rapidly initiated. However, the arsenite contribution remained small while the DMA contribution increased with time. Red cell arsenic included macromolecular arsenic (AsP) as well as three low-molecular-weight forms. The contribution of DMA remained small, but arsenite and AsP contributions increased with time. These findings identify the maternal blood concentration and chemical status of arsenic following the administration of a teratogenic dose of arsenate during the period of organogenesis. They could prove useful for predicting the likelihood of a teratogenic outcome in other mammalian species.
我们测定了妊娠第8天上午腹腔注射致畸剂量的放射性标记砷酸钠后0.2至6小时内怀孕仓鼠血液中砷的浓度和化学成分。注射后0.20小时，血浆和红细胞中均存在砷。注射后0.5小时左右，血浆砷浓度最高达到220微摩尔/千克血液。血浆砷完全以低分子量形式存在。注射后0.20小时，血浆中同时存在亚砷酸盐和二甲基砷酸盐（DMA），这表明砷酸盐还原和砷的甲基化反应迅速启动。然而，亚砷酸盐的占比仍然较小，而DMA的占比随时间增加。红细胞砷包括大分子砷（AsP）以及三种低分子量形式。DMA的占比仍然较小，但亚砷酸盐和AsP的占比随时间增加。这些发现确定了在器官发生期给予致畸剂量砷酸盐后母体血液中砷的浓度和化学状态。它们可能有助于预测其他哺乳动物物种出现致畸结果的可能性。
