Shamloo-Dashtpagerdi Roohollah, Shahriari Amir Ghaffar, Tahmasebi Aminallah, Vetukuri Ramesh R
Department of Agriculture and Natural Resources, Higher Education Center of Eghlid, Eghlid, Iran.
Department of Agriculture, Minab Higher Education Center, University of Hormozgan, Bandar Abbas, Iran.
Front Plant Sci. 2023 May 30;14:1161245. doi: 10.3389/fpls.2023.1161245. eCollection 2023.
MicroRNA (miRNA)-target gene modules are essential components of plants' abiotic stress signalling pathways Little is known about the drought-responsive miRNA-target modules in wheat, but systems biology approaches have enabled the prediction of these regulatory modules and systematic study of their roles in responses to abiotic stresses. Using such an approach, we sought miRNA-target module(s) that may be differentially expressed under drought and non-stressed conditions by mining Expressed Sequence Tag (EST) libraries of wheat roots and identified a strong candidate (). We then assessed molecular and physiochemical differences between two wheat genotypes with contrasting drought tolerance in a controlled drought experiment and assessed possible relationships between their tolerance and evaluated traits. We found that the module significantly responds to drought stress in wheat roots. It is differentially expressed between the contrasting wheat genotypes and under drought versus non-stressed conditions. We also found significant associations between the module's expression profiles and ABA hormone content, water relations, photosynthetic activities, HO levels, plasma membrane damage, and antioxidant enzyme activities in wheat. Collectively, our results suggest that a regulatory module consisting of and may play an important role in wheat's drought tolerance.
微小RNA（miRNA）-靶基因模块是植物非生物胁迫信号通路的重要组成部分。关于小麦中干旱响应性miRNA-靶模块知之甚少，但系统生物学方法已能够预测这些调控模块，并对其在非生物胁迫响应中的作用进行系统研究。利用这种方法，我们通过挖掘小麦根的表达序列标签（EST）文库，寻找可能在干旱和非胁迫条件下差异表达的miRNA-靶模块，并确定了一个强有力的候选模块。然后，我们在控制干旱实验中评估了两种耐旱性相反的小麦基因型之间的分子和生理化学差异，并评估了它们的耐受性与评估性状之间的可能关系。我们发现该模块在小麦根中对干旱胁迫有显著响应。它在耐旱性相反的小麦基因型之间以及干旱与非胁迫条件下差异表达。我们还发现该模块的表达谱与小麦中的脱落酸激素含量、水分关系、光合活性、过氧化氢水平、质膜损伤和抗氧化酶活性之间存在显著关联。总体而言，我们的结果表明，由[具体内容缺失]和[具体内容缺失]组成的调控模块可能在小麦的耐旱性中发挥重要作用。
