从红树林湿地中分离并进行基因组学分析十种新物种。

Isolation and genomics of ten novel species from mangrove wetland.

机构信息

Agricultural Bio-Resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou City, Fujian Province, 350003, PR China.

Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou City, Fujian Province, 350002, PR China.

出版信息

Int J Syst Evol Microbiol. 2023 Jun;73(6). doi: 10.1099/ijsem.0.005929.

DOI:10.1099/ijsem.0.005929

PMID:37327059

Abstract

Mangrove bacteria largely compose the microbial community of the coastal ecosystem and are directly associated with nutrient cycling. In the present study, 12 Gram-negative and motile strains were isolated from a mangrove wetland in Zhangzhou, China. Pairwise comparisons (based on 16S rRNA gene sequences) and phylogenetic analysis indicated that these 12 strains belong to the genus . The 16S rRNA gene sequence similarities among the 12 strains and their related type strains ranged from 98.8 to 99.8 %, but they still could not be considered as known species. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between the 12 strains and their related type strains were below the cut-off values (ANI 95-96% and dDDH 70 %) for prokaryotic species delineation. The DNA G+C contents of the present study strains ranged from 44.4 to 53.8 %. The predominant menaquinone present in all strains was MK-7. The present study strains (except FJAT-53532) also contained ubiquinones (Q-8 and Q-7). The polar lipid phosphatidylglycerol and fatty acid iso-C was noticed in all strains. Based on phenotypic, chemotaxonomic, phylogenetic and genomic comparisons, we propose that these 12 strains represent 10 novel species within the genus , with the names sp. nov. (FJAT-53749=GDMCC 1.2398=KCTC 82649), sp. nov. (FJAT-52072=MCCC 1K05363=KCTC 82447), sp. nov. (FJAT-53764=GDMCC 1.2349=KCTC 82648), sp. nov. (FJAT-53870=GDMCC 1.2346= KCTC 82640), sp. nov. (FJAT-53555=GDMCC 1.2344=KCTC 82645), sp. nov. (FJAT-53532=GDMCC 1.2343=KCTC 82644), sp. nov. (FJAT-54031=GDMCC 1.2347=KCTC 82642), sp. nov. (FJAT-53681=GDMCC 1.2345=KCTC 82641), sp. nov. (FJAT-51860=GDMCC 1.2342=KCTC 82650) and sp. nov. (FJAT-51754=GDMCC 1.2341= KCTC 82647).

摘要

从中国漳州市红树林湿地中分离到 12 株革兰氏阴性、运动型菌株，属于属。基于 16S rRNA 基因序列的系统发育分析和两两比较表明，这 12 株菌属于属。12 株菌与其相关模式菌株的 16S rRNA 基因序列相似性为 98.8%99.8%，但仍不能视为已知种。菌株与相关模式菌株的数字 DNA-DNA 杂交（dDDH）和平均核苷酸同一性（ANI）值均低于细菌种间界定的 cutoff 值（ANI 95%-96%和 dDDH 70%）。本研究菌株的 DNA G+C 含量为 44.4%53.8%。所有菌株的主要类异戊二烯醌均为 MK-7。除了 FJAT-53532 外，其余菌株还含有泛醌（Q-8 和 Q-7）。所有菌株均含有极性脂磷脂酰甘油和脂肪酸 iso-C。基于表型、化学分类、系统发育和基因组比较，我们提出这 12 株菌代表了属中的 10 个新种，其名称分别为 sp. nov.（FJAT-53749=GDMCC 1.2398=KCTC 82649）、 sp. nov.（FJAT-52072=MCCC 1K05363=KCTC 82447）、 sp. nov.（FJAT-53764=GDMCC 1.2349=KCTC 82648）、 sp. nov.（FJAT-53870=GDMCC 1.2346=KCTC 82640）、 sp. nov.（FJAT-53555=GDMCC 1.2344=KCTC 82645）、 sp. nov.（FJAT-53532=GDMCC 1.2343=KCTC 82644）、 sp. nov.（FJAT-54031=GDMCC 1.2347=KCTC 82642）、 sp. nov.（FJAT-53681=GDMCC 1.2345=KCTC 82641）、 sp. nov.（FJAT-51860=GDMCC 1.2342=KCTC 82650）和 sp. nov.（FJAT-51754=GDMCC 1.2341= KCTC 82647）。