Liu Yang, Pei Tao, Du Juan, Zhu Honghui
Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.
Microorganisms. 2023 Nov 27;11(12):2870. doi: 10.3390/microorganisms11122870.
A new, facultatively anaerobic, light-yellow, and rod-shaped bacterium designated as 3B26 isolated from Qi'ao Island's tidal flat sediment was identified. Strain 3B26 can hydrolyze gelatin, aesculin, and skim milk. The major cellular fatty acids were identified as iso-C, referred to as summed feature 3, and C; the polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, and phospholipid; and the quinones contained Q-7, Q-8, MK-7, and MMK7. The genomic size of strain 3B26 was 4,682,650 bp, and its genomic DNA G + C content was 54.8%. While a 16S rRNA gene-based phylogenetic analysis confirmed that strain 3B26 belongs to the genus , both phylogenomic inference and genomic comparison revealed that strain 3B26 is distinguishable from its relatives, and digital DNA-DNA hybridization (dDDH) values of 24.4-62.6% and average nucleotide identities (ANIs) of 83.5-95.6% between them were below the 70% dDDH and 96% ANI thresholds for bacterial species delineation. Genomic functional analysis demonstrated that strain 3B26 possesses complete gene clusters of eicosapentaenoic acid biosynthesis and denitrification. Based on the evidence above, strain 3B26 is considered to represent a novel species of the genus , and the name sp. nov. (type strain 3B26 = GDMCC 1.2057 = KCTC 82339) is proposed.
从淇澳岛潮滩沉积物中分离出一种新的兼性厌氧、浅黄色杆状细菌，命名为3B26。菌株3B26能够水解明胶、七叶苷和脱脂乳。主要的细胞脂肪酸被鉴定为异-C（称为总和特征3）和C；极性脂质由磷脂酰乙醇胺、磷脂酰甘油和磷脂组成；醌类包含Q-7、Q-8、MK-7和MMK7。菌株3B26的基因组大小为4,682,650 bp，其基因组DNA的G + C含量为54.8%。基于16S rRNA基因的系统发育分析证实菌株3B26属于该属，而系统基因组推断和基因组比较表明菌株3B26与其亲缘种不同，它们之间的数字DNA-DNA杂交（dDDH）值为24.4 - 62.6%，平均核苷酸同一性（ANI）为83.5 - 95.6%，均低于细菌物种划分的70% dDDH和96% ANI阈值。基因组功能分析表明菌株3B26拥有二十碳五烯酸生物合成和反硝化的完整基因簇。基于上述证据，菌株3B26被认为代表该属的一个新物种，并提出名称 新种（模式菌株3B26 = GDMCC 1.2057 = KCTC 82339）。
