正常和病理状态下人体血液样本中的内毒素灭活活性。
Endotoxin-inactivating activity in normal and pathological human blood samples.
作者信息
Obayashi T, Tamura H, Tanaka S, Ohki M, Takahashi S, Kawai T
出版信息
Infect Immun. 1986 Aug;53(2):294-7. doi: 10.1128/iai.53.2.294-297.1986.
Abstract
The endotoxin-specific chromogenic test revealed that plasma endotoxin-inactivating activity was markedly diminished by endotoxemia, but not by fungemia or by dialysis with cellulose membranes, suggesting that fungal polysaccharides and other nonendotoxic, Limulus-reactive materials do not consume endotoxin-inactivating factors in the blood. There was a close negative correlation between plasma endotoxin concentration and endotoxin-inactivating activity. The specificity of the test was improved by fractionating amebocyte lysate and using only the factors that constitute the endotoxin-sensitive coagulation pathway of the horseshoe crab. This test was able to differentiate endotoxemia from fungemia and from contamination with other nonendotoxic, Limulus-reactive materials.
摘要
内毒素特异性显色试验显示，内毒素血症可使血浆内毒素灭活活性显著降低，但真菌血症或用纤维素膜透析则不会，这表明真菌多糖及其他非内毒素性、鲎试剂反应性物质不会消耗血液中的内毒素灭活因子。血浆内毒素浓度与内毒素灭活活性之间存在密切的负相关。通过对鲎试剂进行分级分离并仅使用构成鲎内毒素敏感凝血途径的因子，提高了该试验的特异性。该试验能够区分内毒素血症与真菌血症以及与其他非内毒素性、鲎试剂反应性物质的污染。
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