Fessia Aluminé, Sartori Melina, Orlando Julieta, Barros Germán, Nesci Andrea
Laboratorio de Ecología Microbiana, Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Universidad Nacional de Río Cuarto, Ruta Nacional 36, Km 601, X5804ZAB Río Cuarto, Córdoba, Argentina. - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
Laboratorio de Ecología Microbiana, Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, Ñuñoa, Santiago, Chile.
Heliyon. 2024 Jun 13;10(12):e32607. doi: 10.1016/j.heliyon.2024.e32607. eCollection 2024 Jun 30.
In the present study, the genomes of EM-A7 and EM-A8 were sequenced and annotated. The Illumina sequencing platform (NovaSeq PE150) was used to sequence the genomic DNA. There were 6 277 054 raw reads for EM-A7, with a Q20 of 97.52 % and 43.78 % GC, and 8 030 262 raw reads for EM-A8, with a Q20 of 97.53 % and 46.21 % GC. Annotation was carried out by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). The strains were classified taxonomically on the basis of an average nucleotide identity analysis (ANI), as well as through a dDDh analysis on the Genome-to-Genome Distance Calculator (GGDC v3.0). The pipeline predicted 4062 protein-coding sequences (CDSs) and 73 RNA genes (62 tRNA and 6 rRNA) for EM-A7, and 3797 protein-coding sequences (CDSs) and 80 RNA genes for EM-A8. These findings enhance our understanding of the two strains' potential as biocontrol agents to manage disease in maize.
在本研究中,对EM-A7和EM-A8的基因组进行了测序和注释。使用Illumina测序平台(NovaSeq PE150)对基因组DNA进行测序。EM-A7有6277054条原始读数,Q20为97.52%,GC含量为43.78%;EM-A8有8030262条原始读数,Q20为97.53%,GC含量为46.21%。注释由NCBI原核生物基因组注释管道(PGAP)进行。通过平均核苷酸同一性分析(ANI)以及在基因组到基因组距离计算器(GGDC v3.0)上进行的dDDh分析,对菌株进行分类学分类。该管道预测EM-A7有4062个蛋白质编码序列(CDS)和73个RNA基因(62个tRNA和6个rRNA),EM-A8有3797个蛋白质编码序列(CDS)和80个RNA基因。这些发现增强了我们对这两种菌株作为控制玉米病害的生物防治剂潜力的理解。