MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, P. R. China.
Langmuir. 2023 Jul 4;39(26):8941-8951. doi: 10.1021/acs.langmuir.3c00628. Epub 2023 Jun 20.
Liquid-liquid phase separation (LLPS) exists widely in living systems, and understanding the working mechanisms of the formed condensed droplets is of great significance for the prevention and treatment of diseases as well as for the development of biomimetic materials. Herein, in this Perspective we try to focus on the reconstructions of biomolecule-based coacervates and outline the associations between the functional components and droplets as well as the physiological and pathological functions associated with coacervates. Coacervates are formed by functional components through weak, multivalent interactions. The interaction strengths that determine coacervate properties such as electability and phase state, which in turn influence the functional components to limit their fluidity, stability, or diffusion coefficients, are specially discussed. At the end of this Perspective, the current challenges are summarized; progress will require our great efforts to reveal the mechanisms of action at the molecular level and then develop biomolecule-based coacervate models with complexity, integration of methods, and intellectualization.
液-液相分离（LLPS）广泛存在于生命体系中，理解所形成的凝聚液滴的工作机制对于疾病的预防和治疗以及仿生材料的开发都具有重要意义。在此，在本观点中，我们试图专注于基于生物分子共凝聚物的重建，并概述功能成分与液滴之间的关联以及与共凝聚物相关的生理和病理功能。共凝聚物是由功能成分通过弱多价相互作用形成的。决定共凝聚物性质（如可萃取性和相态）的相互作用强度，进而影响功能成分限制其流动性、稳定性或扩散系数，这些内容被专门讨论。在本观点的最后，总结了当前的挑战；取得进展需要我们付出巨大努力，以揭示分子水平上的作用机制，然后开发具有复杂性、方法集成和智能化的基于生物分子的共凝聚物模型。
