Green Christopher M, Sementa Deborah, Mathur Divita, Melinger Joseph S, Deshpande Priyasha, Elbaum-Garfinkle Shana, Medintz Igor L, Ulijn Rein V, Díaz Sebastián A
Center for Bio/Molecular Science and Engineering Code 6900, U.S. Naval Research Laboratory, Washington, DC, 20375, USA.
Nanoscience Initiative at Advanced Science Research Center, Graduate Center of the City University of New York, New York, NY, 10031, USA.
Commun Chem. 2024 Feb 29;7(1):49. doi: 10.1038/s42004-024-01124-3.
Peptide-based liquid-liquid phase separated domains, or coacervates, are a biomaterial gaining new interest due to their exciting potential in fields ranging from biosensing to drug delivery. In this study, we demonstrate that coacervates provide a simple and biocompatible medium to improve nucleic acid biosensors through the sequestration of both the biosensor and target strands within the coacervate, thereby increasing their local concentration. Using the well-established polyarginine (R) - ATP coacervate system and an energy transfer-based DNA molecular beacon we observed three key improvements: i) a greater than 20-fold reduction of the limit of detection within coacervates when compared to control buffer solutions; ii) an increase in the kinetics, equilibrium was reached more than 4-times faster in coacervates; and iii) enhancement in the dye fluorescent quantum yields within the coacervates, resulting in greater signal-to-noise. The observed benefits translate into coacervates greatly improving bioassay functionality.
基于肽的液-液相分离结构域，即凝聚物，作为一种生物材料正重新引起人们的兴趣，因为它们在从生物传感到药物递送等领域具有令人兴奋的潜力。在本研究中，我们证明凝聚物提供了一种简单且生物相容的介质，通过将生物传感器和靶链隔离在凝聚物中来改善核酸生物传感器，从而提高它们的局部浓度。使用成熟的聚精氨酸（R）-ATP凝聚物系统和基于能量转移的DNA分子信标，我们观察到三个关键改进：i）与对照缓冲溶液相比，凝聚物中的检测限降低了20倍以上；ii）动力学增加，凝聚物中达到平衡的速度快了4倍以上；iii）凝聚物中染料荧光量子产率提高，导致信噪比更高。观察到的这些益处转化为凝聚物极大地改善了生物测定功能。
