BIOTARGEN UR7450, Université de Caen Normandie, Caen, France; RESPE, 3 rue Nelson Mandela, Saint Contest, France; ONIRIS, CISCO-ONIRIS, Route de Gachet, Nantes, Cedex, France.
BIOTARGEN UR7450, Université de Caen Normandie, Caen, France; LABÉO, 1 route de Rosel, Saint Contest, France.
J Equine Vet Sci. 2023 Sep;128:104869. doi: 10.1016/j.jevs.2023.104869. Epub 2023 Jun 18.
A total of 752 horses were involved in the CES Valencia Spring Tour 2021. Due to an equine herpesvirus-1 (EHV-1) outbreak, the competition was cancelled and the site was locked down. The objective of this study was to describe epidemiological, clinical, diagnostic, and outcome data of the 160 horses remaining in Valencia. Clinical and quantitative polymerase chain reaction (qPCR) data were analysed for 60 horses in a retrospective case-control observational study. The risk of developing clinical manifestations was explored using a logistic regression approach. EHV-1 was detected by qPCR, genotyped as A2254 (ORF30) and isolated on cell culture. From the 60 horses, 50 (83.3%) showed fever, 30 horses (50%) showed no further signs and 20 (40%) showed neurological signs, with eight horses (16%) hospitalised, of which two died (3%). Stallions and geldings were six times more likely to develop EHV-1 infection compared to mares. Horses older than 9 years, or housed in the middle of the tent were more likely to develop EHV-1 myeloencephalopathy (EHM). These data show that for EHV-1 infection, the risk factor was male sex. For EHM the risk factors were age > 9-year old and location in the middle of the tent. These data highlight the crucial role of stable design, position, and ventilation in EHV-outbreaks. It also showed that PCR testing of the horses was important to manage the quarantine.
共有 752 匹马参加了 2021 年 CES 巴伦西亚春季巡回赛。由于马疱疹病毒 1 型（EHV-1）爆发，比赛被取消，场地被封锁。本研究的目的是描述在巴伦西亚剩余的 160 匹马的流行病学、临床、诊断和结果数据。对 60 匹马进行了回顾性病例对照观察研究，分析了临床和定量聚合酶链反应（qPCR）数据。使用逻辑回归方法探讨了发生临床症状的风险。使用 qPCR 检测 EHV-1，对 ORF30 进行 A2254 （ORF30）基因分型，并在细胞培养物上分离。在 60 匹马中，50 匹（83.3%）出现发热，30 匹（50%）无进一步症状，20 匹（40%）出现神经症状，8 匹（16%）住院，其中 2 匹死亡（3%）。公马和骟马比母马感染 EHV-1 的可能性高 6 倍。年龄大于 9 岁或位于帐篷中央的马更有可能发生 EHV-1 脑脊髓炎（EHM）。这些数据表明，EHV-1 感染的危险因素是性别为雄性。对于 EHM，危险因素是年龄> 9 岁和位于帐篷中央。这些数据突出表明了稳定设计、位置和通风在 EHV 爆发中的重要作用。它还表明对马匹进行 PCR 检测对于管理隔离非常重要。
