铁皮石斛多糖通过抑制铁死亡改善血管性痴呆大鼠认知功能障碍
Dendrobium Nobile Polysaccharides Attenuates Ferroptosis and Improves Cognitive Function in Vascular Dementia Rats.
机构信息
Department of Neurology, the First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, China.
Department of Neurology, People's Hospital of Ganxian District, Ganzhou, Jiangxi, China.
出版信息
Am J Alzheimers Dis Other Demen. 2023 Jan-Dec;38:15333175231185236. doi: 10.1177/15333175231185236.
INTRODUCTION
To investigate the neuroprotective effect and mechanism of polysaccharide (DNP) on vascular dementia (VD) rats.
METHODS
VD model rats were prepared by permanent ligation of bilateral common carotid arteries. Cognitive function was tested by morris water maze test, mitochondrial morphology and ultrastructure of hippocampal synapses were tested by transmission electron microscopy, GSH, xCT, GPx4, and PSD-95 expressions were tested by western blot and PCR.
RESULTS
The number of platform crossing was significantly increased, and the escape latency was significantly shorter in DNP group. The expressions of GSH, xCT and GPx4 in the hippocampus were up-regulated in DNP group. Moreover, the synapses of DNP group were relatively intact and synaptic vesicles increased, the length of synaptic active zone and PSD thickness were significantly increased, and PSD-95 protein expression was significantly up-regulated compared VD group.
CONCLUSION
DNP may take a neuroprotective effect by inhibiting ferroptosis in VD.
简介
为了研究多糖（DNP）对血管性痴呆（VD）大鼠的神经保护作用及其机制。
方法
通过永久性结扎双侧颈总动脉制备 VD 模型大鼠。通过 Morris 水迷宫试验测试认知功能，通过透射电子显微镜测试海马突触的线粒体形态和超微结构，通过 Western blot 和 PCR 测试 GSH、xCT、GPx4 和 PSD-95 的表达。
结果
DNP 组平台穿越次数明显增加，逃避潜伏期明显缩短。DNP 组海马 GSH、xCT 和 GPx4 的表达上调。此外，DNP 组的突触相对完整，突触小泡增多，突触活性区长度和 PSD 厚度明显增加，与 VD 组相比 PSD-95 蛋白表达明显上调。
结论
DNP 可能通过抑制 VD 中的铁死亡发挥神经保护作用。
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