Grunert G, Porcia M, Tchernitchin A N
J Endocrinol. 1986 Jul;110(1):103-14. doi: 10.1677/joe.0.1100103.
The present study describes the effects of oestradiol-17 beta and diethylstilboestrol (DES) on several oestrogenic responses in the immature rat uterus Diethylstilboestrol was weaker than oestradiol in inducing uterine eosinophilia, water imbibition and mitoses, as strong as oestradiol in eliciting epithelial hypertrophy at 24 h after treatment, and stronger than oestradiol in eliciting the reduction of epithelial cell height at 6 h after treatment and myometrial cell hypertrophy at 24 h after treatment. In addition, differences among the mitotic responses to oestrogen of the different uterine cell types were also detected. The above dissociation of the effects of DES and oestradiol-17 beta is in agreement with the hypothesis that eosinophil-mediated non-genomic responses, genomic responses and cell proliferation are mediated by independent mechanisms, involving different receptors which may have different affinities for both compounds. The eosinopenia and eosinophil degranulation under DES treatment suggest an explanation for the effect of DES on water imbibition. The dissociation among genomic responses from the different uterine cell types supports the hypothesis that different kinds of cytosol-nuclear oestrogen receptors exist.
本研究描述了17β-雌二醇和己烯雌酚（DES）对未成熟大鼠子宫几种雌激素反应的影响。己烯雌酚在诱导子宫嗜酸性粒细胞增多、水分吸收和有丝分裂方面比雌二醇弱，在治疗后24小时引起上皮肥大方面与雌二醇一样强，在治疗后6小时引起上皮细胞高度降低和治疗后24小时引起子宫肌层细胞肥大方面比雌二醇更强。此外，还检测到不同子宫细胞类型对雌激素的有丝分裂反应之间的差异。DES和17β-雌二醇作用的上述分离与以下假设一致，即嗜酸性粒细胞介导的非基因组反应、基因组反应和细胞增殖由独立机制介导，涉及对这两种化合物可能具有不同亲和力的不同受体。DES治疗下的嗜酸性粒细胞减少和嗜酸性粒细胞脱颗粒为DES对水分吸收的作用提供了解释。不同子宫细胞类型的基因组反应之间的分离支持了存在不同种类的胞质-核雌激素受体这一假设。
