Grunert G, Tchernitchin A N
J Endocrinol. 1982 Sep;94(3):307-15. doi: 10.1677/joe.0.0940307.
Different effects of oestrogen are mediated separately, by independent mechanisms of action. Accordingly, it has been shown that it is possible to dissociate these effects under various conditions which stimulate or inhibit responses selectively. The present study describes the action of progesterone on non-genomic responses to oestrogen stimulation which, it has been suggested, are mediated by eosinophils. Oestrogen induced an increase in the number of eosinophils in the rat uterus, an increase in uterine wet weight and oedema of deep endometrial stroma (measured as decrease in cellular density). Progesterone induced a slight oedematous reaction in superficial endometrial stroma but did not induce uterine eosinophilia. Progesterone pretreatment of oestrogen-treated rats did not block oestrogen-induced uterine eosinophilia and oedema but induced an increase in the degranulation of uterine eosinophils. Therefore the number of eosinophils decreased without any change in the uterine oedema induced after 6h of oestrogen action. The results corroborate previously published evidence of a dissociation of the action of oestrogen in independent groups of responses. Progesterone, a known blocker of some oestrogenic responses, did not block the effects mediated by eosinophils at 6h after oestrogen administration; any change in these effects at later times can be explained by an earlier eosinophil degranulation under the action of progesterone.
雌激素的不同作用是通过独立的作用机制分别介导的。因此，研究表明，在各种选择性刺激或抑制反应的条件下，可以使这些作用分离。本研究描述了孕酮对雌激素刺激的非基因组反应的作用，有人提出，这些反应是由嗜酸性粒细胞介导的。雌激素可使大鼠子宫内嗜酸性粒细胞数量增加、子宫湿重增加以及深层子宫内膜基质水肿（以细胞密度降低来衡量）。孕酮可使浅表子宫内膜基质出现轻微水肿反应，但不会引起子宫嗜酸性粒细胞增多。对经雌激素处理的大鼠进行孕酮预处理，不会阻断雌激素诱导的子宫嗜酸性粒细胞增多和水肿，但会使子宫嗜酸性粒细胞的脱颗粒增加。因此，嗜酸性粒细胞数量减少，而雌激素作用6小时后诱导的子宫水肿没有任何变化。这些结果证实了先前发表的关于雌激素作用在独立反应组中分离的证据。孕酮是已知的一些雌激素反应的阻断剂，在给予雌激素6小时后，它不会阻断由嗜酸性粒细胞介导的作用；这些作用在后期的任何变化都可以用孕酮作用下早期嗜酸性粒细胞脱颗粒来解释。
