二维铁基金属有机框架纳米片具有过氧化物酶模拟活性，可用于比色检测与虾新鲜度相关的次黄嘌呤。

Two dimensional iron metal-organic framework nanosheet with peroxidase-mimicking activity for colorimetric detection of hypoxanthine related to shrimp freshness.

机构信息

College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China.

出版信息

Talanta. 2023 Dec 1;265:124833. doi: 10.1016/j.talanta.2023.124833. Epub 2023 Jun 19.

DOI:10.1016/j.talanta.2023.124833

Abstract

Two dimensional iron metal-organic framework nanosheet (2D Fe MOF) was facilely synthesized at room temperature by simple stirring of iron salts and terephthalic acid ligand in a mixed solution containing triethylamine. Its morphology and structure were fully characterized by TEM, AFM, XPS and TEM element mapping. Then, its peroxidase-mimicking activity was studied by using HO and 3, 3', 5, 5'- tetramethylbenzidine as substrate. K and V of 2D Fe MOF towards HO were 0.02 mM and 2.08 × 10 M s, respectively. Through the formation of cascade reaction between xanthine oxidase and 2D Fe MOF, a visual method for hypoxanthine (Hx) detection was constructed to evaluate aquatic products freshness. After effective validation, this method presented wide linear range (5.0-500.0 μM), low limit of detection (3.29 μM), satisfied accuracy (recovery of 94.78-99.85%), and good selectivity. By using this method, Hx content in shrimp samples at different storage time were determined.

摘要

二维铁金属有机骨架纳米片（2D Fe MOF）在室温下通过铁盐和对苯二甲酸配体在含有三乙胺的混合溶液中简单搅拌即可轻松合成。通过 TEM、AFM、XPS 和 TEM 元素映射充分表征了其形态和结构。然后，使用 HO 和 3,3',5,5'-四甲基联苯胺作为底物研究了其过氧化物酶模拟活性。2D Fe MOF 对 HO 的 K 和 V 分别为 0.02 mM 和 2.08×10 M s。通过黄嘌呤氧化酶和 2D Fe MOF 之间的级联反应的形成，构建了一种用于检测次黄嘌呤（Hx）的可视化方法，以评估水产品的新鲜度。经过有效验证，该方法呈现较宽的线性范围（5.0-500.0 μM）、较低的检测限（3.29 μM）、令人满意的准确性（回收率为 94.78-99.85%）和良好的选择性。使用该方法，测定了不同贮藏时间虾样品中的 Hx 含量。