School of Traditional Chinese Materia Medica, Key Laboratory for TCM Material Basis Study and Innovative Drug Development of Shenyang City, Shenyang Pharmaceutical University, Shenyang, 110016, PR China; Key Laboratory of Bioresource Research and Development of Liaoning Province, College of Life and Health Sciences, Northeastern University, Shenyang, 110169, China; School of Food and Drug, Luoyang Normal University, Luoyang, 471934, China.
School of Traditional Chinese Materia Medica, Key Laboratory for TCM Material Basis Study and Innovative Drug Development of Shenyang City, Shenyang Pharmaceutical University, Shenyang, 110016, PR China.
Phytochemistry. 2023 Sep;213:113767. doi: 10.1016/j.phytochem.2023.113767. Epub 2023 Jun 20.
Research on natural inhibitors of microglial overactivation derived from members of the Wikstroemia genus revealed that the extract of W. lichiangensis W. W. Sm. Has a remarkable inhibitory effect on nitric oxide production in overactivated microglia. In the present study, thirty-four compounds, including five undescribed sesquiterpenoids [wiksdauctins A-B (1-2) and wikscarotins A-C (3-5)] and one undescribed lignan [wikstroeminasin A (8)], were isolated from a 95% EtOH extract of W. lichiangensis roots using bio-guided phytochemical research. The structures of the isolated compounds were elucidated using comprehensive spectroscopic analyses. Furthermore, their anti-neuroinflammatory effects were evaluated in lipopolysaccharide-stimulated BV-2 microglia. Seventeen isolated compounds exhibited stronger inhibitory effects than positive control minocycline (IC values of 67.08 ± 1.95 μM), with IC values ranging from 7.35 ± 2.51 to 64.49 ± 3.38 μM. The findings of this study imply that the isolated compounds might serve as potential therapeutic agents for neurodegenerative diseases.
从紫薇属植物中提取的小胶质细胞过度激活的天然抑制剂的研究表明，岭南山竹子 W. lichiangensis W. W. Sm. 的提取物对过度激活的小胶质细胞中一氧化氮的产生具有显著的抑制作用。在本研究中，从岭南山竹子根的 95%乙醇提取物中，采用生物导向的植物化学研究方法，分离得到了 34 种化合物，包括 5 种未描述的倍半萜[wiksdauctins A-B（1-2）和 wikscarotins A-C（3-5）]和 1 种未描述的木质素[wikstroeminasin A（8）]。通过综合光谱分析阐明了分离化合物的结构。此外，还评估了它们在脂多糖刺激的 BV-2 小胶质细胞中的抗炎作用。17 种分离得到的化合物的抑制作用强于阳性对照米诺环素（IC 值为 67.08±1.95μM），IC 值范围为 7.35±2.51 至 64.49±3.38μM。本研究的结果表明，这些分离得到的化合物可能是治疗神经退行性疾病的潜在治疗剂。
