School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China.
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China.
J Pharm Biomed Anal. 2023 Sep 20;234:115532. doi: 10.1016/j.jpba.2023.115532. Epub 2023 Jun 19.
Traditional Chinese medicine (TCM) decoction is a complex polydispersed phase system containing colloid solution, emulsion and suspension, which maybe induced by the supramolecular phenomenon in decoction. However, until now there is no systematic analysis of composition and formation mechanism of supramolecules in TCM decoction contained mineral drug and herb medicines. Maxing Shigan Decoction (MXSGT), one of the classic TCM recipes, has been widely used in the treatment of fever in clinic. In this study, we obtained the supramolecular part of MXSGT (MXSGT NPs). And its traceability, formation mechanism, metal and organic components were further analyzed. The morphology was characterized by scanning electron microscopy (SEM) and dynamic light scattering (DLS); and the lipopolysaccharides (LPS) induced rats' fever model was established to evaluate the antipyretic effect of MXSGT NPs. Furthermore, interaction of the disassembled groups was studied to explore the traceability and formation mechanism of MXSGT NPs by isothermal titration calorimeter (ITC). Due to the combination of mineral gypsum and herb medicines, both ICP-OES and UHPLC-Q-Orbitrap HRMS were used to analyze metal and organic components of MXSGT and MXSGT NPs, respectively. The results showed that MXSGT NPs was regular spherical nanoparticles and had the same antipyretic effect as MXSGT. Moreover, MXSGT NPs was formed by the interaction between metal and organic components, resulted in enriching the main active compounds of MXSGT. This study would provide a new idea of studying TCM decoction, especially clarifying the connotation with the participation of mineral gypsum.
中药汤剂是一种复杂的多分散相体系，包含胶体溶液、乳状液和悬浮液，这可能是由汤剂中的超分子现象引起的。然而，直到现在，对于含矿物药和草药的中药汤剂中超分子的组成和形成机制还没有系统的分析。麻杏石甘汤（MXSGT）是一种经典的中药方剂，已广泛应用于临床发热的治疗。在本研究中，我们获得了 MXSGT 的超分子部分（MXSGT NPs）。并进一步分析了其示踪性、形成机制、金属和有机成分。通过扫描电子显微镜（SEM）和动态光散射（DLS）对其形态进行了表征；并建立了 LPS 诱导的大鼠发热模型，以评价 MXSGT NPs 的解热作用。此外，通过等温滴定量热仪（ITC）研究了拆分组的相互作用，以探讨 MXSGT NPs 的示踪性和形成机制。由于矿物石膏和草药的结合，我们分别使用电感耦合等离子体发射光谱法（ICP-OES）和超高效液相色谱-四极杆-Orbitrap 高分辨率质谱法（UHPLC-Q-Orbitrap HRMS）分析 MXSGT 和 MXSGT NPs 的金属和有机成分。结果表明，MXSGT NPs 为规则的球形纳米颗粒，具有与 MXSGT 相同的解热作用。此外，MXSGT NPs 是由金属和有机成分的相互作用形成的，导致 MXSGT 的主要活性化合物得到富集。本研究为研究中药汤剂提供了一个新的思路，特别是澄清了参与矿物石膏的内涵。
