Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei, Anhui 230031, China.
Wanbei Branch, Anhui Academy of Agricultural Sciences, Fuyang, Anhui 236600, China.
Virus Res. 2023 Sep;334:199141. doi: 10.1016/j.virusres.2023.199141. Epub 2023 Jun 24.
Lily plants (Lilium lancifolium Thunb.) exhibiting dwarfing and foliar chlorosis with mosaic or mottle disease symptoms were found in Anhui Province, China. We used high-throughput sequencing of small RNA to survey the virus in the lily cultivation region of Anhui Province. Here, we report the identification and complete genome sequence of the viral agent. It contains 9733 nucleotides, excluding the poly(A) tail, and encodes a polyprotein of 3063 amino acids. The complete polyprotein ORF shows 98.92% amino acid sequence identity with that of iris potyvirus A (GenBank MH898493). Phylogenetic analysis of coat protein sequences placed the viral agent close to members of the genus Potyvirus in the family Potyviridae, and it was therefore provisionally named iris potyvirus A isolate Anhui (IrPVA-Anhui). This is the first complete genome sequence of IrPVA-Anhui from lily plant, for which only a partial sequence from Iris domestica has been reported previously. Comparative analysis of this genome sequence with those of closely related potyviruses identified nine cleavage sites and the conserved motifs typical of potyviruses. Subsequent virus identification was performed using serological assays (ELISA and antibody-based lateral flow assays), molecular methods (RT-PCR), and a pathogenicity test. Virus particles with a length of about 700 nm, similar to viruses in the genus Potyvirus, were observed via transmission electron microscope (TEM). We back-inoculated healthy plants of multiple species to investigate the host range of the virus. It infected the original host, Iris domestica, and Nicotiana benthamiana but not Triticum aestivum, Pisum sativum, Chenopodium amaranticolor, or Datura stramonium. This is the first report of natural IrPVA-Anhui infection of lily plants in China, providing a scientific basis for IrPVA-Anhui control in future lily plantings.
在中国安徽省发现了表现出矮化和叶片黄化、伴有花叶或斑驳症状的百合植株。我们使用高通量测序的小 RNA 来调查安徽省百合种植区的病毒。在这里，我们报告了病毒的鉴定和完整基因组序列。它包含 9733 个核苷酸，不包括 poly(A)尾巴，并编码一个 3063 个氨基酸的多聚蛋白。完整的多聚蛋白 ORF 与虹膜马铃薯 Y 病毒 A（GenBank MH898493）的氨基酸序列同一性为 98.92%。衣壳蛋白序列的系统发育分析将病毒置于马铃薯 Y 病毒科马铃薯 Y 病毒属的成员附近，因此该病毒被暂时命名为鸢尾马铃薯 Y 病毒 A 安徽分离株（IrPVA-Anhui）。这是从百合植物中获得的 IrPVA-Anhui 的第一个完整基因组序列，此前仅从鸢尾属植物中报道了部分序列。对该基因组序列与亲缘关系密切的马铃薯 Y 病毒的比较分析确定了九个切割位点和马铃薯 Y 病毒特有的保守基序。随后通过血清学检测（ELISA 和基于抗体的侧向流动检测）、分子方法（RT-PCR）和致病性试验进行病毒鉴定。通过透射电子显微镜（TEM）观察到长度约为 700nm 的病毒粒子，类似于马铃薯 Y 病毒属的病毒。我们对多种健康植物进行了回接接种，以研究病毒的宿主范围。它感染了原宿主鸢尾属植物和烟草，但不感染小麦、豌豆、苋菜或曼陀罗。这是中国首例自然感染百合植物的 IrPVA-Anhui 报告，为今后百合种植中 IrPVA-Anhui 的防治提供了科学依据。
