State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, 530004, China.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, 530004, China.
Poult Sci. 2023 Aug;102(8):102815. doi: 10.1016/j.psj.2023.102815. Epub 2023 May 29.
Germ cell loss is a crucial biological event during germ cell development. The number of female germ cells determines the reproductive performance and egg production of hens. Various intrinsic and extrinsic factors affect germ cell loss, such as germ cell nest breakdown in early life and nutritional deficiencies during daily husbandry. Here, we examined the effect of fasting on the germ cell number of chicks. The results showed that 72 h fasting resulted in a higher germ cell loss than that by 24 h fasting in chicks. The RNA-seq analysis revealed that the genes of ribosome pathway were down-regulated and the biological processes of protein processing in endoplasmic reticulum were inhibited in starved chicks. Furthermore, in female chicks treated with 72 h fasting, the qPCR of ovaries showed down-regulation of ribosome-related genes, and transmission electron microscopy imaging of ovaries showed fewer ribosomes. The blood biochemical indices indicated that 72 h fasting reduced the liver functions and affected the glucose metabolism, lipid metabolites and ion metabolites. In summary, the present results concluded negative impacts on the germ cell pool by prolonged fasting in the early life of chicks and manifested that adequate management should be cared for fasted time for breeding.
生殖细胞丢失是生殖细胞发育过程中的一个关键生物学事件。雌性生殖细胞的数量决定了母鸡的繁殖性能和产蛋量。各种内在和外在因素都会影响生殖细胞丢失，例如早期生活中生殖细胞巢的破裂以及日常饲养中的营养缺乏。在这里，我们研究了禁食对雏鸡生殖细胞数量的影响。结果表明，与禁食 24 h 相比，禁食 72 h 会导致雏鸡的生殖细胞丢失更多。RNA-seq 分析显示，饥饿雏鸡中核糖体途径的基因下调，内质网中蛋白质加工的生物学过程受到抑制。此外，在接受 72 h 禁食处理的雌性雏鸡中，卵巢的 qPCR 显示核糖体相关基因下调，卵巢的透射电镜成像显示核糖体减少。血液生化指标表明，72 h 禁食会降低肝功能并影响葡萄糖代谢、脂质代谢物和离子代谢物。综上所述，本研究结果表明，早期生活中长时间禁食会对生殖细胞库产生负面影响，并表明在育雏期间应注意禁食时间的适当管理。
