School of Dentistry, Epigenetics Nanodiagnostic and Therapeutic Group, Center for Orofacial Regeneration, Rehabilitation and Reconstruction (COR3), The University of Queensland, Brisbane, Australia.
School of Dentistry, The University of Queensland, Brisbane, Australia.
Tissue Eng Part C Methods. 2023 Jul;29(7):298-306. doi: 10.1089/ten.TEC.2023.0051.
This cross-sectional pilot study explored extracellular vesicle (EV)-derived gene expression of markers for bone turnover and pro-inflammatory cytokines in periodontal disease. Whole unstimulated saliva was collected from 52 participants (18 healthy, 13 gingivitis, and 21 stages III/IV periodontitis), from which salivary small extracellular vesicles (sEVs) were enriched using the size-exclusion chromatography method, and characterized by morphology, EV-protein, and size distribution, using transmission electron microscopy (TEM), enzyme-linked immunosorbent assay (ELISA), and Nanoparticle Tracking Analysis (NTA), respectively. Bone turnover markers and pro-inflammatory cytokines in salivary sEVs were evaluated using reverse transcription PCR. Salivary sEVs morphology, mode, size distribution, and particle concentration were comparable between healthy, gingivitis, and periodontitis patients. The CD9+ subpopulation was significantly higher in periodontitis-derived salivary sEVs compared with healthy. The detection of sEVs mRNA for and was significantly decreased and increased, respectively, in periodontitis compared with healthy controls, with good discriminatory power for periodontitis diagnosis (area under the curve >0.72). This pilot study demonstrated that salivary sEVs mRNAs may serve as a potential noninvasive biomarker source for periodontitis diagnosis.
本横断面初步研究探讨了牙周病中外泌体（EV）衍生的骨转换标志物和促炎细胞因子的基因表达。从 52 名参与者（18 名健康者、13 名牙龈炎患者和 21 名牙周炎 III/IV 期患者）中采集未刺激全唾液，使用排阻色谱法从唾液中小细胞外囊泡（sEVs）中富集，使用透射电子显微镜（TEM）、酶联免疫吸附试验（ELISA）和纳米颗粒跟踪分析（NTA）分别对 EV 蛋白和大小分布进行形态学、EV-蛋白和大小分布的特征分析。使用逆转录 PCR 评估唾液 sEVs 中的骨转换标志物和促炎细胞因子。健康、牙龈炎和牙周炎患者的唾液 sEVs 形态、模式、大小分布和颗粒浓度相似。与健康对照组相比，牙周炎来源的唾液 sEVs 中的 CD9+亚群显著升高。与健康对照组相比，牙周炎患者唾液 sEVs 中 和 的检测显著降低和升高，对牙周炎诊断具有良好的鉴别能力（曲线下面积>0.72）。本初步研究表明，唾液 sEVs mRNAs 可能成为牙周炎诊断的一种潜在非侵入性生物标志物来源。
