Goswami Kangkan Jyoti, Sen Sarma Neelotpal
Advanced Materials Laboratory, Institute of Advanced Study in Science and Technology, Paschim Boragaon, Guwahati 781035, Assam, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
ACS Omega. 2023 Jun 6;8(24):21914-21928. doi: 10.1021/acsomega.3c01753. eCollection 2023 Jun 20.
The abuse of tetracycline (TC) antibiotics causes the accumulation of their residue in the environment, which has an irreversible impact on food safety and human health. In light of this, it is vital to offer a portable, quick, efficient, and selective sensing platform to detect TC instantly. Herein, we have successfully developed a sensor using silk fibroin-decorated thiol-branched graphene oxide quantum dots through a well-known thiol-ene click reaction. It is applied to ratiometric fluorescence sensing of TC in real samples in the linear range of 0-90 nM, with the detection limit of 49.69, 47.76, 55.25, 47.90, and 45.78 nM for deionized water, chicken sample, fish sample, human blood serum, and honey sample, respectively. With the gradual addition of TC to the liquid media, the sensor develops a synergetic luminous effect in which the fluorescence intensity of the nanoprobe steadily declines at 413 nm, while the intensity of a newly emerging peak increases at 528 nm, maintaining a ratio that is dependent on the analyte concentration. The increase of luminescence properties in the liquid media is clearly visible by naked eyes in the presence of 365 nm UV light. The result helps us in building a filter paper strip-based portable smart sensor using an electric circuit comprising a 365 nm LED (light-emitting diode) powered by a mobile phone battery which is attached just below the rear camera of a smartphone. The camera of the smartphone captures the color changes that occur throughout the sensing process and translates into readable RGB data. The dependency of color intensity with respect to the concentration of TC was evaluated by deducing a calibration curve from where the limit of detection was calculated and found to be 0.125 μM. These kinds of gadgets are important for the possible real-time, on-the-spot, quick detection of analytes in situations where high-end approaches are not easily accessible.
四环素（TC）类抗生素的滥用导致其残留物在环境中积累，这对食品安全和人类健康产生不可逆转的影响。有鉴于此，提供一个便携式、快速、高效且具有选择性的传感平台以即时检测TC至关重要。在此，我们通过著名的硫醇-烯点击反应成功开发了一种使用丝素蛋白修饰的硫醇支化氧化石墨烯量子点的传感器。它应用于实际样品中TC的比率荧光传感，线性范围为0 - 90 nM，对于去离子水、鸡肉样品、鱼肉样品、人血清和蜂蜜样品，检测限分别为49.69、47.76、55.25、47.90和45.78 nM。随着向液体介质中逐渐添加TC，该传感器产生协同发光效应，其中纳米探针在413 nm处的荧光强度稳步下降，而新出现的峰在528 nm处的强度增加，保持一个依赖于分析物浓度的比率。在365 nm紫外光存在下，液体介质中发光特性的增加肉眼清晰可见。该结果有助于我们构建一种基于滤纸条的便携式智能传感器，其使用由手机电池供电的包含365 nm发光二极管（LED）的电路，该电路附着在智能手机后置摄像头下方。智能手机的摄像头捕捉整个传感过程中发生的颜色变化并转化为可读的RGB数据。通过推导校准曲线评估颜色强度与TC浓度的相关性，从该校准曲线计算出检测限为0.125 μM。这类装置对于在高端方法不易获得的情况下可能进行的实时、现场、快速分析物检测非常重要。
