Key Laboratory of Ecology and Environment in Minority Areas (Minzu University of China), National Ethnic Affairs Commission, Beijing, 100081, China; College of Life and Environmental Sciences, Minzu University of China, Beijing, 100081, China.
Key Laboratory of Ecology and Environment in Minority Areas (Minzu University of China), National Ethnic Affairs Commission, Beijing, 100081, China; College of Life and Environmental Sciences, Minzu University of China, Beijing, 100081, China.
Plant Physiol Biochem. 2023 Aug;201:107845. doi: 10.1016/j.plaphy.2023.107845. Epub 2023 Jun 22.
Bryophyllum pinnatum (Lam.) Oken is an ornamental and ethno-medicine plant, which can grow a circle of adventitious bud around the leaf margin. The dynamic change of metabolites during the development of B. pinnatum remains poorly understood. Here, leaves from B. pinnatum at four developmental stages were sampled based on morphological characteristics. A non-targeted metabolomics approach was used to evaluate the changes of endogenous metabolites during adventitious bud formation in B. pinnatum. The results showed that differential metabolites were mainly enriched in sphingolipid metabolism, flavone and flavonol biosynthesis, phenylalanine metabolism, and tricarboxylic acid cycle pathway. The metabolites assigned to amino acids, flavonoids, sphingolipids, and the plant hormone jasmonic acid decreased from period Ⅰ to Ⅱ, and then increased from period Ⅲ to Ⅳ with the emergence of adventitious bud (period Ⅲ). While the metabolites related to the tricarboxylic acid cycle showed a trend of first increasing and then decreasing during the four observation periods. Depending on the metabolite changes, leaves may provide conditions similar to in vitro culture for adventitious bud to occur, thus enabling adventitious bud to grow at the leaf edge. Our results provide a basis for illustrating the regulatory mechanisms of adventitious bud in B. pinnatum.
落地生根（Lam.）Oken 是一种观赏和民族药用植物，其叶片边缘可环绕生长一圈不定芽。然而，其不定芽发育过程中代谢物的动态变化仍知之甚少。本研究基于形态特征，从四个发育阶段的落地生根叶片中取样，采用非靶向代谢组学方法评估了不定芽形成过程中内源代谢物的变化。结果表明，差异代谢物主要富集在鞘脂代谢、黄酮和黄酮醇生物合成、苯丙氨酸代谢和三羧酸循环途径。分配到氨基酸、类黄酮、鞘脂和植物激素茉莉酸的代谢物从第Ⅰ期到第Ⅱ期减少，然后在不定芽出现的第Ⅲ期到第Ⅳ期增加（第Ⅲ期）。而与三羧酸循环相关的代谢物在四个观察期内呈现先增加后减少的趋势。根据代谢物的变化，叶片可能为不定芽的发生提供类似于体外培养的条件，从而使不定芽在叶片边缘生长。本研究结果为阐明落地生根不定芽的调控机制提供了依据。
