CINBIO, Universidade de Vigo, Deparment of Chemical Engineering, Campus Ourense, Edificio Politécnico, As Lagoas s/n, 32004 Ourense, Spain; IMT Mines Albi, Allée des Sciences, 81000 Albi, France.
CINBIO, Universidade de Vigo, Deparment of Chemical Engineering, Campus Ourense, Edificio Politécnico, As Lagoas s/n, 32004 Ourense, Spain.
Bioresour Technol. 2021 Dec;342:125882. doi: 10.1016/j.biortech.2021.125882. Epub 2021 Sep 6.
Microwave hydrothermal processing was employed to obtain valuable gelling or bioactive fractions from U. pinnatifida, assessing the processing conditions following a biorefinery concept. It was identified a relevant impact on the antioxidant properties, sulfate, protein and oligosaccharides content, with the highest values above 200 °C, although the maximum in fucose was obtained at 160 °C. The lowest temperature involved the highest minerals and sulfate content of the solid phases. Rheology indicated that hydrothermal treatment at 160 °C is adequate to extract alginates with structural and viscoelastic properties similar to those commercially available. The incorporation of the hydrothermal residual solids in the proposed alginate matrices favored the development of systems with potential non-food applications applications. Selected extracts, after an intensification stage using ultrasound, featured interesting biological activities for two human cancer cell lines (A2780; HeLa 229) with percentage of cellular inhibition > 83 and 57%, without positive effects on A549 and HCT-116.
采用微波水热法从 U. pinnatifida 中获得有价值的胶凝或生物活性部分，按照生物炼制概念评估加工条件。结果表明，抗氧化性能、硫酸盐、蛋白质和低聚糖含量均受到显著影响，在 200°C 以上时含量最高，而岩藻糖含量在 160°C 时达到最高。最低温度下固相中矿物质和硫酸盐含量最高。流变学研究表明，在 160°C 下水热处理可以充分提取具有类似结构和粘弹性的海藻酸盐。将水热残余固体掺入所提出的海藻酸盐基质中有利于开发具有潜在非食品应用的系统。经过超声强化阶段的选定提取物对两种人类癌细胞系（A2780；HeLa 229）具有有趣的生物活性，细胞抑制率均>83%和 57%，对 A549 和 HCT-116 没有积极影响。
