Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Temuco 4811230, Chile.
Carrera de Biotecnología, Facultad de Ciencias Agropecuarias y Medioambiente, Universidad de La Frontera, Temuco 4811230, Chile.
Int J Mol Sci. 2024 Sep 29;25(19):10499. doi: 10.3390/ijms251910499.
, present in the microbiota of human skin and nasal mucosa, has recently emerged as a causative agent of hospital-acquired infections, notable for its resistance to multiple antimicrobials. Its mobilome comprises several mobile genetic elements, such as plasmids, transposons, insertion sequences and integrons, which contribute to the acquisition of antimicrobial resistance genes. This study analyzes the contribution of the mobilome in the transfer and dissemination of resistance genes. In addition, integrative and conjugative elements (ICEs), essential in the dissemination of resistance genes between bacterial populations, whose role in has not yet been studied, are examined. This study examined 365 genomes obtained from the NCBI Pathogen Detection database. Phylogenetic and pangenome analyses were performed, the resistance profile of the bacterium was recognized, and mobile elements, including putative ICE, were detected. Bioinformatic analyses identified 20 antimicrobial resistance genes in this species, with the gene being the most predominant. Resistance genes were mainly associated with plasmid sequence regions and class 1 integrons. Although an ICE was detected, no resistance genes linked to this element were found. This study provided valuable information on the geographic spread and prevalence of outbreaks observed through phylogenetic and pangenome analyses, along with identifying antimicrobial resistance genes and mobile genetic elements that carry many of the resistance genes and may be the subject of future research and therapeutic approaches.
，存在于人类皮肤和鼻腔黏膜的微生物群中，最近已成为医院获得性感染的病原体，其特点是对多种抗菌药物具有耐药性。其移动组包括几种可移动的遗传元件，如质粒、转座子、插入序列和整合子，这些元件有助于获得抗菌药物耐药基因。本研究分析了移动组在耐药基因的转移和传播中的作用。此外，还研究了整合子和共轭元件（ICEs），它们在细菌种群之间传播耐药基因方面起着重要作用，而在 中的作用尚未得到研究。本研究检查了从 NCBI 病原体检测数据库获得的 365 个基因组。进行了系统发育和泛基因组分析，识别了该细菌的耐药谱，并检测了移动元件，包括推定的 ICE。生物信息学分析在该物种中鉴定出 20 种抗菌药物耐药基因，其中 基因最为普遍。耐药基因主要与质粒序列区域和 1 类整合子相关。尽管检测到了一个 ICE，但没有发现与该元件相关的耐药基因。本研究通过系统发育和泛基因组分析提供了有关该物种地理传播和流行情况的有价值信息，同时还鉴定了抗菌药物耐药基因和携带许多耐药基因的可移动遗传元件，这些可能是未来研究和治疗方法的主题。
