Wu Zijuan, Yan Yanyun, Li Wenli, Li Yali, Yang Huansheng
Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan Normal University, No. 36 Lushan Road, Changsha 410081, China.
Animals (Basel). 2023 Jun 14;13(12):1979. doi: 10.3390/ani13121979.
Early weaning stress impairs intestinal health in piglets. miRNAs are crucial for maintaining host homeostasis, while their implication for animal health remains unclear. To identify weaning-associated miRNAs, piglets were sampled at day 0, 1, 3, 7 and 14 after weaning. The data indicated that the highest levels of miR-199a-5p in jejunal villus upper cells were observed on day 14 after weaning, while the lowest levels in crypt cells were noted on day 7 and 14. In contrast, miR-199a-3p was down-regulated in both of these two cells on day 7 after weaning compared with day 0. Both miR-199a-5p and -3p were differently expressed along the villus-crypt axis. To further clarify the function of miR-199a, mice deficient in miR-199a were exposed to dextran sulfate sodium (DSS) to induce colitis. Results revealed that silencing of miR-199a enhanced sensitivity to DSS-induced colitis. Moreover, the increased morbidity and mortality were correlated with enhanced inflammatory cell infiltration, elevated pro-inflammatory cytokine expression, impaired barrier function, and a concomitant increase in permeability-related parameters. Bioinformatic analysis further demonstrated that lipid metabolism-related pathways were significantly enriched and Ndrg1 was verified as a target of miR-199a-3p. These findings indicate that miR-199a may be important for animal health management.
早期断奶应激会损害仔猪的肠道健康。微小RNA（miRNAs）对于维持宿主内环境稳态至关重要，但其对动物健康的影响仍不清楚。为了鉴定与断奶相关的miRNAs，在断奶后第0、1、3、7和14天对仔猪进行采样。数据表明，断奶后第14天在空肠绒毛上部细胞中观察到最高水平的miR-199a-5p，而在第7天和第14天隐窝细胞中观察到最低水平。相比之下，与第0天相比，断奶后第7天这两种细胞中的miR-199a-3p均下调。miR-199a-5p和-3p沿绒毛-隐窝轴的表达均存在差异。为了进一步阐明miR-199a的功能，将缺乏miR-199a的小鼠暴露于葡聚糖硫酸钠（DSS）以诱导结肠炎。结果显示，miR-199a的沉默增强了对DSS诱导的结肠炎的敏感性。此外，发病率和死亡率的增加与炎症细胞浸润增强、促炎细胞因子表达升高、屏障功能受损以及通透性相关参数的相应增加有关。生物信息学分析进一步表明，脂质代谢相关途径显著富集，并且Ndrg1被证实为miR-199a-3p的靶标。这些发现表明，miR-199a可能对动物健康管理很重要。
