Bradic Jovana, Andjic Marijana, Novakovic Jovana, Kocovic Aleksandar, Tomovic Marina, Petrovic Anica, Nikolic Marina, Mitrovic Slobodanka, Jakovljevic Vladimir, Pecarski Danijela
Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia.
Center of Excellence for Redox Balance Research in Cardiovascular and Metabolic Disorders, Svetozara Makovica 69, 34000 Kragujevac, Serbia.
Antioxidants (Basel). 2023 Jun 15;12(6):1277. doi: 10.3390/antiox12061277.
This study aimed to examine the effects of a 14-day treatment with lady's bedstraw methanol extract on doxorubicin-induced cardiotoxicity through functional, biochemical and histological examinations. We used 24 male rats divided into the following groups: control (CTRL), doxorubicin (DOX), and DOX + GVE ( extract). GVE was administered orally at a dose of 50 mg/kg per day for 14 days, while a single dose of doxorubicin was injected into the DOX groups. After accomplishing treatment with GVE, cardiac function was assessed, which determined the redox state. During the autoregulation protocol on the Langendorff apparatus, ex vivo cardiodynamic parameters were measured. Our results demonstrated that the consumption of GVE effectively suppressed the disturbed response of the heart to changes in perfusion pressures caused by administration of DOX. Intake of GVE was associated with a reduction in most of the measured prooxidants in comparison to the DOX group. Moreover, this extract was capable of increasing the activity of the antioxidant defense system. Morphometric analyses showed that rat hearts treated with DOX showed more pronounced degenerative changes and necrosis compared to the CTRL group. However, GVE pretreatment seems to be able to prevent the pathological injuries caused by DOX injection via decrease in oxidative stress and apoptosis.
本研究旨在通过功能、生化和组织学检查，探讨14天的蓬子菜甲醇提取物治疗对阿霉素诱导的心脏毒性的影响。我们使用了24只雄性大鼠，分为以下几组：对照组（CTRL）、阿霉素组（DOX）和DOX + GVE（提取物）组。GVE以每天50 mg/kg的剂量口服给药，持续14天，而DOX组则注射单剂量的阿霉素。在用GVE完成治疗后，评估心脏功能，这确定了氧化还原状态。在Langendorff装置上的自动调节实验过程中，测量离体心脏动力学参数。我们的结果表明，GVE的使用有效地抑制了心脏对阿霉素给药引起的灌注压力变化的紊乱反应。与DOX组相比，GVE的摄入与大多数测量的促氧化剂的减少有关。此外，这种提取物能够增加抗氧化防御系统的活性。形态计量分析表明，与CTRL组相比，用阿霉素治疗的大鼠心脏表现出更明显的退行性变化和坏死。然而，GVE预处理似乎能够通过降低氧化应激和细胞凋亡来预防阿霉素注射引起的病理损伤。
